PXD015392

PXD015392 is an original dataset announced via ProteomeXchange.

Title	Degron flexibility enforces auxin sensing
Description	Cullin RING-type E3 ubiquitin ligase SCFTIR1/AFB1-5 and their ubiquitylation targets, AUX/IAAs, sense auxin concentrations in the nucleus. TIR1 binds a surface- exposed degron in AUX/IAAs promoting their ubiquitylation and rapid auxin-regulated proteasomal degradation. Here, we resolved TIR1·auxin·IAA7 and TIR1·auxin·IAA12 complex topology, and show that flexible intrinsically disordered regions (IDRs) cooperatively position AUX/IAAs on TIR1. The AUX/IAA PB1 interaction domain also assists in non-native contacts, affecting AUX/IAA dynamic interaction states. Our results establish a role for IDRs in modulating auxin receptor assemblies. By securing AUX/IAAs on two opposite surfaces of TIR1, IDR diversity supports locally tailored positioning for targeted ubiquitylation, and might provide conformational flexibility for adopting a multiplicity of functional states. We postulate IDRs in distinct members of the AUX/IAA family to be an adaptive signature for protein interaction and initiation region for proteasome recruitment.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:34:06.837.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015392
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Wolfgang Hoehenwarter
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-10 02:46:00	ID requested
⏵ 1	2024-10-07 10:34:07	announced

10.1038/s41467-020-16147-2;

Niemeyer M, Moreno Castillo E, Ihling CH, Iacobucci C, Wilde V, Hellmuth A, Hoehenwarter W, Samodelov SL, Zurbriggen MD, Kastritis PL, Sinz A, Calder, ó, n Villalobos LIA, Flexibility of intrinsically disordered degrons in AUX/IAA proteins reinforces auxin co-receptor assemblies. Nat Commun, 11(1):2277(2020) [pubmed]

10.6019/PXD015392;

curator keyword: Biological

submitter keyword: degron, auxin, phytohormones, AUX/IAA, SCFTIR1,intrinsic disorder, allostery

Wolfgang Hoehenwarter
contact affiliation	Proteome Analytics, Leibniz Institute of Plant Biochemistry
contact email	wolfgang.hoehenwarter@ipb-halle.de
lab head
Wolfgang Hoehenwarter
contact affiliation	Leibniz Institute of Plant Biochemistry
contact email	wolfgang.hoehenwarter@ipb-halle.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD015392

PRIDE project URI

• PRIDE
  1. PXD015392
     1. Label: PRIDE project
     2. Name: Degron flexibility enforces auxin sensing