PXD015384

PXD015384 is an original dataset announced via ProteomeXchange.

Title	TNP1 and TNP2 mRNA binding proteins from mouse testes
Description	We hypothesized that specific proteins associate with Tnp mRNAs to suppress or stimulate their translation. We took a proteomics approach and developed a method to isolate endogenous mRNA species and their associated proteins. We employed biotinylated DNA oligonucleotides and avidin resin to capture endogenous Tnp mRNAs and associated molecules from testis extract, and we specifically eluted the complexes with non-biotinylated oligonucleotides. We then used mass spectrometry to identify proteins that were associated with the Tnp1 and Tnp2 mRNAs but not Actin mRNAs. We hypothesize that the proteins that are specifically enriched by Tnp1 and Tnp2 mRNA pulldowns relative to Actin mRNA pulldown may function to regulate these transcripts.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:58:34.075.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jason Williams
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue
Instrument	6340 Ion Trap LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-10 01:38:59	ID requested
1	2019-10-04 01:49:55	announced
⏵ 2	2024-10-22 04:58:35	announced	2024-10-22: Updated project metadata.

10.1038/s41598-019-50052-z;

Phillips BT, Williams JG, Atchley DT, Xu X, Li JL, Adams AL, Johnson KL, Hall TMT, Mass spectrometric identification of candidate RNA-binding proteins associated with Transition Nuclear Protein mRNA in the mouse testis. Sci Rep, 9(1):13618(2019) [pubmed]

submitter keyword: spermatocytes, mouse,RNA binding proteins

Jason G. Williams
contact affiliation	Epigenetics and Stem Cell Biology Laboratory; National Institute of Environmental Health Sciences; National Institutes of Health
contact email	willia56@niehs.nih.gov
lab head
Jason Williams
contact affiliation	National Institute of Environmental Health Sciences
contact email	willia56@niehs.nih.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015384
     1. Label: PRIDE project
     2. Name: TNP1 and TNP2 mRNA binding proteins from mouse testes