PXD015378 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | UFMylation maintains translational homeostasis to ensure cell cycle progression |
Description | Cellular homeostasis is tightly linked to proliferation ensuring that only healthy cells divide. Homeostasis-sensing pathways including mTOR and integrated stress response (ISR) employ phosphorylation to regulate translation initiation and consequently cell cycle progression. Whether ubiquitin or ubiquitin-like molecules impact on translation and proliferation as part of existing or novel pathways is unknown. Here, we combine cell cycle screening, mass spectrometry and ribosome profiling to elucidate the molecular mechanism by which UFMylation, the modification of proteins with ubiquitin-fold modifier 1 molecules, controls translational homeostasis and cell cycle progression. Perturbation of UFMylation prevents eIF4F translation initiation complex assembly and recruitment of the ribosome. The ensuing global translational shutdown is sensed by cyclin D1 and halts the cell cycle independently of canonical ISR and mTOR signaling. Our findings establish UFMylation as a key regulator of translation that employs the principle of conserved cellular sensing mechanisms to couple translational homeostasis to cell cycle progression. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-30 |
AnnouncementXML | Submission_2022-05-30_07:53:41.541.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | James Wright |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-09-09 07:32:28 | ID requested | |
⏵ 1 | 2022-05-30 07:53:41 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: TMT, MSMS, ubiquitylation, |
Contact List
Jyoti Choudhary |
contact affiliation | Institute of Cancer Research, London & Wellcome Trust Sanger Institute |
contact email | jyoti.choudhary@icr.ac.uk |
lab head | |
James Wright |
contact affiliation | Functional Proteomics, Institute Cancer Research & Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute |
contact email | jw13@sanger.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015378
- Label: PRIDE project
- Name: UFMylation maintains translational homeostasis to ensure cell cycle progression