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PXD015359

PXD015359 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCell-surface proteomics identifies differences in signalling and adhesion protein expression between naive and primed human pluripotent stem cells
DescriptionNaive and primed human pluripotent stem cells (hPSC) provide valuable models to study cellular and molecular developmental processes. The lack of detailed information about cell-surface protein expression in these two pluripotent cell types prevents an understanding of how the cells communicate and interact with their microenvironments. Here, we used plasma membrane profiling to directly measure cell-surface protein expression in naive and primed hPSC. This unbiased approach quantified over 1700 plasma membrane proteins including those involved in cell adhesion, signalling and cell interactions. Notably, multiple cytokine receptors upstream of JAK-STAT signalling were more abundant in naive hPSC. In addition, functional experiments showed that FOLR1 and SUSD2 proteins are highly expressed at the cell surface in naive hPSC but are not required to establish human naive pluripotency. This study provides a comprehensive stem cell proteomic resource that uncovers differences in signalling pathway activity and has identified new markers to define human pluripotent states.
HostingRepositoryPRIDE
AnnounceDate2020-04-17
AnnouncementXMLSubmission_2020-04-21_22:32:12.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKatarzyna Wojdyla
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-09-09 02:50:43ID requested
12020-04-16 22:17:05announced
22020-04-21 22:32:13announced2020-04-22: Updated publication reference for PubMed record(s): 32302559.
Publication List
Wojdyla K, Collier AJ, Fabian C, Nisi PS, Biggins L, Oxley D, Rugg-Gunn PJ, Cell-Surface Proteomics Identifies Differences in Signaling and Adhesion Protein Expression between Naive and Primed Human Pluripotent Stem Cells. Stem Cell Reports, 14(5):972-988(2020) [pubmed]
Keyword List
submitter keyword: plasma membrane profilling, naive and primed human pluripotent stem cells
Contact List
Peter Rugg-Gunn
contact affiliationEpigenetics Programme, The Babraham Institute, Cambridge, UK and Wellcome Trust - MRC Cambridge Stem Cell Institute, Cambridge, UK
contact emailpeter.rugg-gunn@babraham.ac.uk
lab head
Katarzyna Wojdyla
contact affiliationUniversity of Cambridge
contact emailwojdyla.kat@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
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