PXD015352

PXD015352 is an original dataset announced via ProteomeXchange.

Title	PTPRR interactome detection using PUP-IT (pupylation-based interaction tagging) Mass Spectrometry
Description	To further stably express PTPRR (WT)-PafA or PTPRR (DA)-PafA in iPUP OVCAR5 cell, we subcloned PTPRR-WT or DA, respectively, into the PafA-IRES-EGFP plasmid. Each plasmid was packed into a lentivirus and then transduced into iPUP OVACR5 cells for 48 h. GFP-positive cells were sorted by flow cytometry. The expression of PTPRR (WT)-PafA and PTPRR (DA)-PafA was confirmed by western blotting analysis. PTPRR (WT)-PafA or PTPRR (DA)-PafA expressed iPUP OVCAR5 cells were grown to a cell density of about 75% on 10 cm dishes. We followed the protocol established previous. To prepare PUP-IT samples for mass spectrometry analysis, including doxycycline induction, biotin labeling, cell lysis, streptavidin magnetic beads pull-down, trypsin digestion, and peptide cleaning.
HostingRepository	PRIDE
AnnounceDate	2019-11-27
AnnouncementXML	Submission_2019-11-27_06:51:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuetong Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-09 01:27:10	ID requested
1	2019-10-30 02:33:38	announced
⏵ 2	2019-11-27 06:51:55	announced	2019-11-27: Updated project metadata.

Wang Y, Cao J, Liu W, Zhang J, Wang Z, Zhang Y, Hou L, Chen S, Hao P, Zhang L, Zhuang M, Yu Y, Li D, Fan G, -catenin. J Biol Chem, 294(48):18306-18323(2019) [pubmed]

submitter keyword: ptprr, lc-ms

Gaofeng Fan
contact affiliation	shanghaitech university
contact email	fangf@shanghaitech.edu.cn
lab head
Yuetong Wang
contact affiliation	shanghaitech university
contact email	wangyt1@shanghaitech.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD015352

PRIDE project URI

• PRIDE
  1. PXD015352
     1. Label: PRIDE project
     2. Name: PTPRR interactome detection using PUP-IT (pupylation-based interaction tagging) Mass Spectrometry