PXD015336

PXD015336 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of differentially expressed proteins in the serum of patients with acute renal allograft rejection using iTRAQ labelling technology
Description	Transplantation is currently the best treatment option for end-stage renal disease (ESRD) patients. However, acute rejection (AR) is the main source of failure in renal transplantation. The current gold standard for diagnosis of AR involves renal biopsy, but it is invasive, time consuming, costly and inconvenient. Sensitive and less invasive detection of AR episodes in renal transplant patients is essential to preserve the allograft function. Here, we applied isobaric tags for relative and absolute quantisation (iTRAQ) mass spectrometry to analyse serum protein expression in AR patients and healthy controls. Overall, 1399 proteins were identified. Using a cut-off of Q<0.05 and a fold change > 1.2 for the expressed variation, 109 proteins showed distinct differential expression between the AR and control groups, of whom 72 were upregulated and 37 were downregulated. Several proteins, such as properdin, keratin 1, lipoprotein (a) and vitamin D-binding protein, may play roles in the pathogenesis of AR. This study focused on iTRAQ-based proteomic profiling of serum samples in AR. Insights from our work might help advance our understanding of the molecular mechanisms of AR and identify potential novel biomarkers of AR for further characterization.
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_14:15:25.003.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yue Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-06 00:37:09	ID requested
⏵ 1	2021-09-08 14:15:25	announced

Zhang Y, Ou M, Lin H, Lai L, Chen H, Chen J, Sui W, Xue W, Zhang R, Gan Q, Tang D, Sun X, Dong J, Yan Q, Dai Y, Proteomic analysis of differentially expressed proteins in the serum of patients with acute renal allograft rejection using iTRAQ labelling technology. Mol Med Rep, 22(3):2329-2341(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: acute rejection, renal allograft, iTRAQ, proteomics, serum

Yong Dai
contact affiliation	1. Nephrology Department of Guilin No. 924 Hospital, Guangxi Key laboratory of Metabolic Diseases Research, Guilin Key laboratory of Kidney Diseases Research, 541002, Guilin, Guangxi, People’s Republic of China. 2. Clinical Medical Research Center, the Second Clinical Medical College of Jinan University (Shenzhen People’s Hospital), 518020, Shenzhen, Guangdong, People’s Republic of China.
contact email	daiyong22@aliyun.com
lab head
Yue Zhang
contact affiliation	Nephrology Department of Guilin No. 924 Hospital, Guangxi Key laboratory of Metabolic Diseases Research, Guilin Key laboratory of Kidney Diseases Research, 541002, Guilin, Guangxi, People’s Republic of China
contact email	wingss0908@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015336
     1. Label: PRIDE project
     2. Name: Proteomic analysis of differentially expressed proteins in the serum of patients with acute renal allograft rejection using iTRAQ labelling technology