PXD015327 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Differential scaling between G1 protein production and cell size dynamics promotes commitment to the cell division cycle in budding yeast |
Description | In the unicellular eukaryote Saccharomyces cerevisiae, Cln3-CDK activity enables Start, the irreversible commitment to the cell division cycle. However, the concentration of Cln3 has been paradoxically considered to remain constant during G1, due to the presumed scaling of its production rate with cell size dynamics. Measuring metabolic and biosynthetic activity during cell cycle progression in single cells, we found that cells exhibit pulses in protein production rate, which do not scale with cell size dynamics, but -following the intrinsic metabolic dynamics- peak around Start. Using a viral-based bicistronic construct and targeted proteomics to measure Cln3 at the single-cell and population level, we show that the differential scaling between protein production and cell size leads to a temporal increase in Cln3 concentration, and passage through Start. This differential scaling causes Start in both daughter and mother cells, across growth conditions. Thus, uncoupling between two fundamental physiological parameters drives cell cycle commitment. |
HostingRepository | PRIDE |
AnnounceDate | 2019-10-29 |
AnnouncementXML | Submission_2019-10-29_09:19:18.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alexander Schmidt |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-09-05 01:38:59 | ID requested | |
⏵ 1 | 2019-10-29 09:19:19 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: yest, cell cycle, cell division |
Contact List
Alexander Schmidt |
contact affiliation | Biozentrum, University of Basel, Switzerland |
contact email | alex.schmidt@unibas.ch |
lab head | |
Alexander Schmidt |
contact affiliation | Proteomics Core Facility |
contact email | alex.schmidt@unibas.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015327
- Label: PRIDE project
- Name: Differential scaling between G1 protein production and cell size dynamics promotes commitment to the cell division cycle in budding yeast