PXD015312

PXD015312 is an original dataset announced via ProteomeXchange.

Title	New insights into amebiasis using a human 3D-intestinal model
Description	Entamoeba histolytica, the agent of amebiasis, colonizes the human colon and can invade the lining of the colon to disseminate in the deep layers of the intestine. Amebiasis mainly affects poor people in developing countries, where the barriers between human feces and food or water are inadequate. Humans are the only reservoir of E. histolytica and are the sole target organism of the development of the disease, which limits our knowledge of the crosstalk between the colon and the parasite, especially during the acute phase of amebiasis. In the present work, we constructed a three-Dimensional (3D)-intestinal model capable of reproducing important features of the human intestine. Using this model and leading-edge technologies, including tissue and cell imaging, transcriptomics, and proteomics, as well as ELISA-based immune response inspection, the early stages of amebic infection have been studied. The data obtained highlight the importance of several virulence markers already shown in patients or experimental models, but also underscore the involvement of other factors that appear to be key regulators of gene expression or important in the secretome of the infected tissue. In addition, we characterized the cellular stress responses against amebiasis and novel regulatory mechanisms utilized by this parasite to modulate the immune response and survive within the human intestine.
HostingRepository	PRIDE
AnnounceDate	2020-03-24
AnnouncementXML	Submission_2020-03-24_08:42:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MARIETTE MATONDO
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Entamoeba histolytica HM-1:IMSS; NCBI TaxID: 294381;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-04 05:11:55	ID requested
⏵ 1	2020-03-24 08:42:35	announced

Aguilar-Rojas A, Castellanos-Castro S, Matondo M, Gianetto QG, Varet H, Sismeiro O, Legendre R, Fernandes J, Hardy D, Copp, é, e JY, Olivo-Marin JC, Guillen N, Insights into amebiasis using a human 3D-intestinal model. Cell Microbiol, 22(8):e13203(2020) [pubmed]

submitter keyword: 3D-intestinal model, mass spectrometry, infection

Nancy Guillen
contact affiliation	Institut Pasteur, Paris, F-75015, France
contact email	mariette.matondo@pasteur.fr
lab head
MARIETTE MATONDO
contact affiliation	Pasteur
contact email	mariette.matondo@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD015312

PRIDE project URI

• PRIDE
  1. PXD015312
     1. Label: PRIDE project
     2. Name: New insights into amebiasis using a human 3D-intestinal model