PXD015304
PXD015304 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | MSstats splitplot method for protein quantitation in MS datasets-SRM controlled mixture |
Description | Digested Escherichia coli protein extract were mixed with 89 different isotopically-labelled reference heavy peptides (5 pmol, 15N2,13C6-Lys, 15N4,13C6-Arg), and with different amounts of the corresponding light peptides (10 pmol, 5 pmol, 2.5 pmol), as 1, 2 and 4, respectively. Light peptides were dived in three different subsets and five different mixtures were prepared in triplicates. SRM measurements for the light-heavy synthetic peptides were performed on a hybrid triple quadrupole/ion trap mass spectrometer (4000 Q-Trap, AB Sciex) equipped with a nano-LC electrospray ionization source. Details are available in description.pdf |
HostingRepository | MassIVE |
AnnounceDate | 2019-09-03 |
AnnouncementXML | Submission_2019-09-03_13:07:04.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Meena Choi |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | No PTMs are included in the dataset |
Instrument | 4000 QTRAP |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-09-03 13:03:17 | ID requested | |
⏵ 1 | 2019-09-03 13:07:05 | announced |
Publication List
no publication |
Keyword List
submitter keyword: MSstats, statistics, mass spectrometry, proteomics, SRM controlled mixture |
Contact List
Eduard Sabido | |
---|---|
contact affiliation | Proteomics Unit, Center for Genomics Regulation, The Barcelona Institute of Science and Technology |
contact email | eduard.sabido@crg.cat |
lab head | |
Meena Choi | |
contact affiliation | Northeastern University |
contact email | mnchoi67@gmail.com |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084220/ |