PXD015296

PXD015296 is an original dataset announced via ProteomeXchange.

Title	Proteomic profile of Cephalosporin-Resistant Neisseria gonorrhoeae Isolates by low pH nano-HPLC-MS/MS
Description	The experiment was performed using three N. gonorrhoeae clinical isolates and N. gonorrhoeae strain ATCC 49226 was used as a reference strain. Tandem mass spectra were processed by PEAKS Studio version 8.5 (Bioinformatics Solutions Inc., Waterloo, Canada). PEAKS DB was set up to search the database assuming trypsin as the digestion enzyme and searched with a fragment ion mass tolerance of 0.05 Da and a parent ion tolerance of 7 ppm. Carbamidomethylation (C) and iTRAQ 4plex (K, N-term) were specified as the fixed modification. Oxidation (M), Deamidation (NQ), and Acetylation (Protein N-term) were specified as the variable modifications. Peptides were filtered with a 1% false discovery rate (FDR) and were required to be a unique peptide. PEAKSQ was used for peptide and protein abundance calculation. Normalization was performed by averaging the abundance of all peptides, and medians were used for averaging.
HostingRepository	PRIDE
AnnounceDate	2019-10-22
AnnouncementXML	Submission_2019-10-22_06:23:36.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015296
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Weiming Gu
SpeciesList	scientific name: Neisseria gonorrhoeae; NCBI TaxID: 485;
ModificationList	iTRAQ4plex; Deamidated; Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-03 06:55:18	ID requested
⏵ 1	2019-10-22 06:23:37	announced

Dataset with its publication pending

submitter keyword: low pH nano-HPLC-MS/MS

Cephalosporin-Resistant Neisseria gonorrhoeae

Weiming Gu
contact affiliation	Shanghai Skin Disease Hospital
contact email	WeimingGu_sh@126.com
lab head
Weiming Gu
contact affiliation	Shanghai Skin Disease Hospital
contact email	WeimingGu_sh@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD015296

PRIDE project URI

• PRIDE
  1. PXD015296
     1. Label: PRIDE project
     2. Name: Proteomic profile of Cephalosporin-Resistant Neisseria gonorrhoeae Isolates by low pH nano-HPLC-MS/MS