PXD015295

PXD015295 is an original dataset announced via ProteomeXchange.

Title	The plastidial Arabidopsis thaliana NFU1 delivers [4Fe-4S] clusters to specific client proteins
Description	Proteins incorporating iron-sulfur (Fe-S) cofactors are required for a plethora of metabolic processes. Their maturation depends on three Fe-S cluster assembly machineries located in the cytosol, mitochondria and chloroplasts. After de novo formation on scaffold proteins, Fe-S centers are loaded on client proteins by use of Fe-S cluster transfer proteins. Among plastidial representatives of this latter category, NFU2 and NFU3 are required for the maturation of the [4Fe-4S] clusters found in photosystem I subunits acting upstream of HCF101. NFU2 is additionally required for the maturation of the [2Fe-2S] dihydroxyacid dehydratase, important for branched-chain amino acid synthesis. Here, we report that recombinant Arabidopsis thaliana NFU1 is able to solely assemble a [4Fe-4S] cluster per homodimer. We also provide insights into the specificity of NFU1 for the maturation of chloroplastic Fe-S proteins by performing co-immunoprecipitation experiments and assaying the physical interaction of NFU1 with many [4Fe-4S]-containing plastidial proteins using binary yeast two-hybrid assays. Interactions with two proteins involved in isoprenoid and thiamine biosynthesis, respectively 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (ISPG) and 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase (THIC), have been further confirmed by bimolecular fluorescence complementation and in vitro Fe-S cluster transfer experiments. The additional interactions detected with SUFD and SUFA allowed building a model in which NFU1 receives its Fe-S cluster from the SUFBC2D scaffold complex and serves for the maturation of [4Fe-4S] client proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:08:29.645.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nathalie Berger
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-03 06:36:19	ID requested
1	2020-07-06 07:30:37	announced
⏵ 2	2024-10-22 05:08:33	announced	2024-10-22: Updated project metadata.

10.1074/jbc.ra119.011034;

Roland M, Przybyla-Toscano J, Vignols F, Berger N, Azam T, Christ L, Santoni V, Wu HC, Dhalleine T, Johnson MK, Dubos C, Couturier J, Rouhier N, NFU1 protein binds and delivers [4Fe-4S] clusters to specific client proteins. J Biol Chem, 295(6):1727-1742(2020) [pubmed]

submitter keyword: arabidopsis

Nicolas Rouhier
contact affiliation	Unite Mixte de Recherches 1136 Université de Lorraine/INRA
contact email	Nicolas.Rouhier@Univ-Lorraine.Fr
lab head
Nathalie Berger
contact affiliation	INRA
contact email	nathalie.berger@inra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD015295

PRIDE project URI

• PRIDE
  1. PXD015295
     1. Label: PRIDE project
     2. Name: The plastidial Arabidopsis thaliana NFU1 delivers [4Fe-4S] clusters to specific client proteins