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PXD015282

PXD015282 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDNMT1 IP-MS alalysis to identify ubiquitin-dependent interacting partners
DescriptionStable inheritance of DNA methylation is critical for maintaining the differentiated phenotypes in multicellular organisms. However, the molecular basis ensuring high fidelity of maintenance DNA methylation is largely unknown. Here, we demonstrate that two distinct modes of DNMT1 recruitment, one is DNA replication-coupled and the other is uncoupled mechanism, regulate the stable inheritance of DNA methylation. PCNA-associated factor 15 (PAF15) represents a primary target of UHRF1 and undergoes dual mono-ubiquitylation (PAF15Ub2) on chromatin. PAF15Ub2 specifically interacts with DNMT1 and controls the recruitment of DNMT1 in a DNA replication-coupled manner. Thus, loss of PAF15Ub2 results in impaired DNA methylation at sites replicating during early S phase. In contrast, outside of S phase or when PAF15 ubiquitylation is perturbed, UHRF1 ubiquitylates histone H3 to promote DNMT1 recruitment. Together, we identify replication-coupled and uncoupled mechanisms of maintenance DNA methylation, both of which collaboratively ensure the stable DNA methylation.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:56:38.737.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAkinori Endo
SpeciesList scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationListubiquitination signature dipeptidyl lysine; phosphorylated residue; acetylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-09-02 05:56:16ID requested
12020-02-25 00:23:57announced
22020-03-24 01:19:41announced2020-03-24: Updated publication reference for PubMed record(s): 32144273.
32024-10-22 04:56:42announced2024-10-22: Updated project metadata.
Publication List
10.1038/s41467-020-15006-4;
Nishiyama A, Mulholland CB, Bultmann S, Kori S, Endo A, Saeki Y, Qin W, Trummer C, Chiba Y, Yokoyama H, Kumamoto S, Kawakami T, Hojo H, Nagae G, Aburatani H, Tanaka K, Arita K, Leonhardt H, Nakanishi M, Two distinct modes of DNMT1 recruitment ensure stable maintenance DNA methylation. Nat Commun, 11(1):1222(2020) [pubmed]
Keyword List
submitter keyword: DNMT1 ubiquitin
Contact List
Atsuya Nishiyama
contact affiliationDivision of Cancer Cell Biology, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan
contact emailanishiya@ims.u-tokyo.ac.jp
lab head
Akinori Endo
contact affiliationTokyo Metropolitan Institute of Medical Science
contact emailendo-ak@igakuken.or.jp
dataset submitter
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Dataset FTP location
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