PXD015282 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | DNMT1 IP-MS alalysis to identify ubiquitin-dependent interacting partners |
Description | Stable inheritance of DNA methylation is critical for maintaining the differentiated phenotypes in multicellular organisms. However, the molecular basis ensuring high fidelity of maintenance DNA methylation is largely unknown. Here, we demonstrate that two distinct modes of DNMT1 recruitment, one is DNA replication-coupled and the other is uncoupled mechanism, regulate the stable inheritance of DNA methylation. PCNA-associated factor 15 (PAF15) represents a primary target of UHRF1 and undergoes dual mono-ubiquitylation (PAF15Ub2) on chromatin. PAF15Ub2 specifically interacts with DNMT1 and controls the recruitment of DNMT1 in a DNA replication-coupled manner. Thus, loss of PAF15Ub2 results in impaired DNA methylation at sites replicating during early S phase. In contrast, outside of S phase or when PAF15 ubiquitylation is perturbed, UHRF1 ubiquitylates histone H3 to promote DNMT1 recruitment. Together, we identify replication-coupled and uncoupled mechanisms of maintenance DNA methylation, both of which collaboratively ensure the stable DNA methylation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:56:38.737.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Akinori Endo |
SpeciesList | scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; |
ModificationList | ubiquitination signature dipeptidyl lysine; phosphorylated residue; acetylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-09-02 05:56:16 | ID requested | |
1 | 2020-02-25 00:23:57 | announced | |
2 | 2020-03-24 01:19:41 | announced | 2020-03-24: Updated publication reference for PubMed record(s): 32144273. |
⏵ 3 | 2024-10-22 04:56:42 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1038/s41467-020-15006-4; |
Nishiyama A, Mulholland CB, Bultmann S, Kori S, Endo A, Saeki Y, Qin W, Trummer C, Chiba Y, Yokoyama H, Kumamoto S, Kawakami T, Hojo H, Nagae G, Aburatani H, Tanaka K, Arita K, Leonhardt H, Nakanishi M, Two distinct modes of DNMT1 recruitment ensure stable maintenance DNA methylation. Nat Commun, 11(1):1222(2020) [pubmed] |
Keyword List
submitter keyword: DNMT1 ubiquitin |
Contact List
Atsuya Nishiyama |
contact affiliation | Division of Cancer Cell Biology, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan |
contact email | anishiya@ims.u-tokyo.ac.jp |
lab head | |
Akinori Endo |
contact affiliation | Tokyo Metropolitan Institute of Medical Science |
contact email | endo-ak@igakuken.or.jp |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015282
- Label: PRIDE project
- Name: DNMT1 IP-MS alalysis to identify ubiquitin-dependent interacting partners