PXD015271

PXD015271 is an original dataset announced via ProteomeXchange.

Title	Disome-seq reveals sequence-mediated coupling of translational pauses and protein structures
Description	The regulation of translation elongation plays a vital role in protein folding; an adequate translational pause provides time and cellular environments for the co-translational folding of nascent peptides. However, the genomic landscape, sequence determinants, and molecular consequences of translational pausing remain mostly unknown. In this study, we performed disome-seq that sequenced mRNA fragments protected by two consecutive ribosomes – a product of severe translational pauses during which the upstream ribosome collides into the paused one. We detected severe translational pauses on ~75% of yeast genes. These pauses were often explained by one of the three mechanisms: 1) slow ribosome releasing at stop codons, 2) slow peptide formation from proline, glycine, asparagine, and cysteine, and 3) slow leaving of polylysine from the exit tunnel of ribosomes. Notably, these amino acids also terminate the α-helical conformation. Such dual roles of amino acids establish an inborn coupling between the synthetic completion of a structural motif and a translational pause. Furthermore, paused ribosomes often recruit chaperones to assist protein folding. As a consequence, emergent protein structures during evolution should be ready to be correctly folded. Collectively, our study shows widespread translational pauses and sheds lights on a better understanding of the regulation of co-translational protein folding.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:16:25.940.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanming Chen
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	6x(13)C labeled residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-02 01:15:34	ID requested
1	2020-12-10 00:11:52	announced
⏵ 2	2024-10-22 05:16:26	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: SILAC,Saccharomyces cerevisiae

Wenfeng Qian
contact affiliation	State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China
contact email	wfqian@genetics.ac.cn
lab head
Yanming Chen
contact affiliation	Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
contact email	ymchen@genetics.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015271
     1. Label: PRIDE project
     2. Name: Disome-seq reveals sequence-mediated coupling of translational pauses and protein structures