PXD015253

PXD015253 is an original dataset announced via ProteomeXchange.

Title	BMP-inhibition in Drosophila secondary cells remodels the seminal proteome, and self and rival ejaculate functions
Description	Seminal fluid proteins (SFPs) exert potent effects on male and female fitness. These rapidly evolving and molecularly diverse proteins derive from multiple male secretory cells and tissues. In Drosophila melanogaster most SFPs are produced in the accessory glands, which are composed of ~1000 fertility-enhancing ‘main cells’ and ~40, more functionally cryptic, ‘secondary cells’. Previous work shows that inhibition of BMP-signalling in secondary cells suppresses secretion, leading to a surprising uncoupling of normal female post-mating responses: refractoriness stimulation is impaired, but offspring production is not. Secondary cell secretions might therefore make a highly specific contribution to the seminal proteome and ejaculate function; alternatively, they might play a more global – but hitherto-undiscovered – role in regulating SFPs and their interconnected functions. Here, we present data that supports the latter model. We show that in addition to previously reported phenotypes, secondary cell-specific BMP-inhibition compromises sperm storage and increases female sperm use efficiency. Moreover, it alters sperm competition: second male sperm enter storage more slowly, ejaculates are ejected later, and first male paternity share improves. This result suggests a novel constraint on ejaculate evolution whereby female refractoriness and sperm competitiveness cannot be simultaneously maximised. Using quantitative proteomics, we reveal changes to the seminal proteome that surprisingly encompass alterations to main cell-derived proteins, indicating important cross-talk between classes of SFP-secreting cells. Our results demonstrate that ejaculate composition and function emerge from the integrated action of multiple secretory cell-types suggesting that modification to the cellular make-up of seminal fluid-producing tissues is an important factor in ejaculate evolution.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:31:14.278.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015253
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Philip Charles
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	Propionamide; Oxidation
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-30 08:22:30	ID requested
⏵ 1	2024-10-07 10:31:19	announced

Hopkins BR, Sepil I, Bonham S, Miller T, Charles PD, Fischer R, Kessler BM, Wilson C, Wigby S, secondary cells remodels the seminal proteome and self and rival ejaculate functions. Proc Natl Acad Sci U S A, 116(49):24719-24728(2019) [pubmed]

10.1073/pnas.1914491116;

10.6019/PXD015253;

submitter keyword: seminal fluid, sperm competition, sexual selection,reproduction

Stuart Wigby
contact affiliation	Edward Grey Institute Department of Zoology University of Oxford
contact email	stuart.wigby@zoo.ox.ac.uk
lab head
Philip Charles
contact affiliation	University of Oxford
contact email	philip.charles@ndm.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015253
     1. Label: PRIDE project
     2. Name: BMP-inhibition in Drosophila secondary cells remodels the seminal proteome, and self and rival ejaculate functions