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PXD015251

PXD015251 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAnalysis of factors bound to native metaphase ESC chromosomes following purification by flow cytometry
DescriptionStem cells are able to self-renew and also generate differentiated progeny. How gene expression patterns are transmitted through mitosis remains unresolved. Conventional studies have relied upon rigorous cell-cycle-stage synchronisation to find factors in mitotic lysates that could ‘bookmark’ the genome. Here we use an alternative approach, purifying native-unfixed mitotic chromosomes from different cell types using flow cytometry and directly identifying chromosome-bound proteins by LC-MS/MS. This revealed a rich profile of pluripotency- and lineage-specific transcription factors that remained bound to mitotic chromosomes in pluripotent ESCs and lymphocytes, as well as factors implicated in chromosome condensation, architecture and gene silencing. Removal of DNA methylation, PRC2 activity, or in situ cleavage of cohesin, each resulted in the reduced compaction of mitotic chromosomes. These data provide a comprehensive catalogue of bookmarking factors in pluripotent versus lineage-restricted cells and demonstrate an expected duality of function by candidates in regulating both gene expression and mitotic chromosome structure.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:07:13.982.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHolger Kramer
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-30 08:19:21ID requested
12020-06-15 03:10:27announced
22020-09-11 03:32:48announced2020-09-11: Updated publication reference for PubMed record(s): 32807789.
32024-10-22 05:07:14announced2024-10-22: Updated project metadata.
Publication List
10.1038/s41467-020-17823-z;
Djeghloul D, Patel B, Kramer H, Dimond A, Whilding C, Brown K, Kohler AC, Feytout A, Veland N, Elliott J, Bharat TAM, Tarafder AK, L, ö, we J, Ng BL, Guo Y, Guy J, Huseyin MK, Klose RJ, Merkenschlager M, Fisher AG, Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction. Nat Commun, 11(1):4118(2020) [pubmed]
Keyword List
submitter keyword: Mitosis, stem cells, chromosomes, chromatin
Contact List
Amanda G. Fisher
contact affiliationLymphocyte Development Group MRC London Institute of Medical Sciences Imperial College School of Medicine Hammersmith Hospital Campus Du Cane Road London, W12 0NN United Kingdom
contact emailamanda.fisher@lms.mrc.ac.uk
lab head
Holger Kramer
contact affiliationMRC London Institute of Medical Sciences
contact emailh.kramer@lms.mrc.ac.uk
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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