PXD015247

PXD015247 is an original dataset announced via ProteomeXchange.

Title	Comparative TMT-based quantitative proteomic analysis of the Tachaea chinensis isopod during parasitism
Description	Parasitic isopods perforate and attach to the host integument via the mandibles, then feed on hemolymph and exudate from the wounds. Such isopods attack a variety of commercially important fish and crustacean hosts. Like other hematophagous parasites, isopods employ biomolecules that inhibit host blood conglutination and defense. In the present study, a tandem mass tag-based quantitative proteomic approach was used to identify differentially expressed proteins in Tachaea chinensis parasites of shrimp, by comparing parasitic (fed) and pre-parasitic (unfed) individuals. We identified 888 proteins from 1510 total peptides, with a significant difference in 129 between the fed and unfed groups. Among these, 37 were upregulated and 92 were downregulated in unfed T. chinensis. This indicates that T. chinensis may require more energy before parasitism during its search for a host. In addition, as is the case for other blood-sucking parasites, it might secrete antihemostatic, anti-inflammatory and immunomodulatory molecules to facilitate blood meal acquisition. Our study is the first to use a TMT-based proteomic approach to analyze the proteome of isopod parasites, and our results will facilitate our understanding of the molecular mechanisms of isopod parasitism on crustaceans.
HostingRepository	PRIDE
AnnounceDate	2020-05-26
AnnouncementXML	Submission_2020-05-26_14:27:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yingdong Li
SpeciesList	scientific name: Tachaea chinensis; NCBI TaxID: 1862870;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-30 05:28:27	ID requested
⏵ 1	2020-05-26 14:27:27	announced

Li Y, Li X, Han Z, Xu W, Li X, Chen Q, Isopod During Parasitism. Front Cell Infect Microbiol, 9():350(2019) [pubmed]

submitter keyword: comparative proteomic, isopod parasite, Tachaea chinensis, blood-sucking, during parasitism

Yingdong Li
contact affiliation	College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China
contact email	liyingdong@syau.edu.cn
lab head
Yingdong Li
contact affiliation	Shenyang Agriculture Universtiy
contact email	liyingdong@syau.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015247
     1. Label: PRIDE project
     2. Name: Comparative TMT-based quantitative proteomic analysis of the Tachaea chinensis isopod during parasitism