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PXD015244

PXD015244 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAcyldepsipeptide probes facilitate specific detection of caseinolytic protease P independent of its oligomeric and activity state
DescriptionCaseinolytic protease P (ClpP) is a tetradecameric peptidase which assembles with chaperones such as ClpX to gain proteolytic activity. Acyldepsipeptides (ADEPs) represent small molecule mimics of ClpX, which bind into hydrophobic pockets on the apical site of the complex and thereby induce activation of ClpP. Detection of ClpP has so far been facilitated with active site directed probes which depend on the activity and oligomeric state of the complex. In order to expand the scope of ClpP labeling, we here introduce a stepwise synthetic approach to customized ADEP photoprobes. Structure-activity relationship studies with small fragments and ADEP derivatives paired with modeling studies revealed design principles of suitable probe molecules. The derivatives were tested for activation of ClpP and subsequently applied in labeling studies of the wild type peptidase as well as enzymes bearing mutations at the active site and an oligomerization sensor. Satisfyingly, the ADEP photoprobes provided a labeling readout of ClpP independent of its activity and oligomeric state.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:05:21.692.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBarbara Hofbauer
SpeciesList scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-30 03:55:30ID requested
12020-05-26 14:31:17announced
22024-10-22 05:05:22announced2024-10-22: Updated project metadata.
Publication List
10.1002/cbic.201900477;
Eyermann B, Meixner M, Br, ö, tz-Oesterhelt H, Antes I, Sieber SA, P Independent of Its Oligomeric and Activity State. Chembiochem, 21(1-2):235-240(2020) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Thermo Fischer Q Exactive Orbitrap,photoaffinity labeling, S. aureus
Contact List
Stephan A. Sieber
contact affiliationDepartment of Chemistry Chair of Organic Chemistry II Technical University of Munich Germany
contact emailstephan.sieber@tum.de
lab head
Barbara Hofbauer
contact affiliationTechnische Universität München
contact emailb.hofbauer@tum.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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