PXD015243

PXD015243 is an original dataset announced via ProteomeXchange.

Title	Proteome Composition of Lysosomes and Autophagosomes after Proteasome Inhibition
Description	Lysosomes are a major site of intracellular acidic hydrolase-mediated proteolysis and cellular degradation in a membrane-enclosed organelle. Alternatively, soluble, ubiquitin-bound proteins are degraded via the proteasome, a megadalton protein complex within the cytoplasm. The interplay between both degradation pathways has been of increasing interest in recent years. To determine the effects of proteasome inhibition on the lysosomal compartment, we investigated enriched lysosomal fractions, autophagosomal fractions and immunoprecipitated proteasomes from HEK293 cells after proteasome inibition by MG132 or bortezomib in comparison to the respective fractions from control cells.
HostingRepository	PRIDE
AnnounceDate	2020-04-20
AnnouncementXML	Submission_2020-04-20_00:03:23.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Melanie Thelen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue; acetylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-30 03:35:57	ID requested
⏵ 1	2020-04-20 00:03:24	announced

Goebel T, Mausbach S, Tuermer A, Eltahir H, Winter D, Gieselmann V, Thelen M, Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7. Mol Cell Proteomics, 19(7):1120-1131(2020) [pubmed]

curator keyword: Biological

submitter keyword: Lysosome, Proteasome, Proteaphagy

Volkmar Gieselmann
contact affiliation	University of Bonn Insitute of Biochemistry and Molecular Biology Nussallee 11 53115 Bonn Germany
contact email	gieselmann@ibmb.uni-bonn.de
lab head
Melanie Thelen
contact affiliation	Institute for Biochemistry and Molecular Biology, University Bonn, Germany
contact email	mthelen@uni-bonn.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD015243

PRIDE project URI

• PRIDE
  1. PXD015243
     1. Label: PRIDE project
     2. Name: Proteome Composition of Lysosomes and Autophagosomes after Proteasome Inhibition