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PXD015234

PXD015234 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNon-reversible tissue fixation by EDC carbodiimide allows for imaging extracellular vesicles in situ
DescriptionExtracellular vesicles (EVs) are secreted nanosized particles with many biological functions and a broad range of pathological associations. A major technical limitation to understanding the role of EVs in normal and diseased specimens has been the inability to visualize the spatial localization of EVs in tissue microenvironments. Here, we use bovine ocular tissue, the vitreous humor, as a model system to study EV imaging. We show that mammalian tissues crosslinked with conventional formaldehyde solutions result in significant EV loss, with subsequent reduced or negative EV signals; however, EV escape can be prevented by additional fixation with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) that permanently holds these nano-sized particles and allows for visualization of EVs in normal and cancer tissues in situ.
HostingRepositoryPRIDE
AnnounceDate2019-11-18
AnnouncementXMLSubmission_2019-11-18_01:16:53.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterhenrik molina
SpeciesList scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationListacetylated residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-30 01:21:56ID requested
12019-11-18 01:16:55announced
Publication List
Gupta MP, Tandalam S, Ostrager S, Lever AS, Fung AR, Hurley DD, Alegre GB, Espinal JE, Remmel HL, Mukherjee S, Levine BM, Robins RP, Molina H, Dill BD, Kenific CM, Tuschl T, Lyden D, D'Amico DJ, Pena JTG, Non-reversible tissue fixation retains extracellular vesicles for in situ imaging. Nat Methods, 16(12):1269-1273(2019) [pubmed]
Keyword List
submitter keyword: imaging extracellular vesicles fixation tissue
Contact List
Pena, John T.G.
contact affiliationWeill Cornell Medicine
contact emailjpena65@gmail.com
lab head
henrik molina
contact affiliationTHE ROCKEFELLER UNIVERSITY
contact emailhenrik.molina@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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