PXD015234 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Non-reversible tissue fixation by EDC carbodiimide allows for imaging extracellular vesicles in situ |
Description | Extracellular vesicles (EVs) are secreted nanosized particles with many biological functions and a broad range of pathological associations. A major technical limitation to understanding the role of EVs in normal and diseased specimens has been the inability to visualize the spatial localization of EVs in tissue microenvironments. Here, we use bovine ocular tissue, the vitreous humor, as a model system to study EV imaging. We show that mammalian tissues crosslinked with conventional formaldehyde solutions result in significant EV loss, with subsequent reduced or negative EV signals; however, EV escape can be prevented by additional fixation with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) that permanently holds these nano-sized particles and allows for visualization of EVs in normal and cancer tissues in situ. |
HostingRepository | PRIDE |
AnnounceDate | 2019-11-18 |
AnnouncementXML | Submission_2019-11-18_01:16:53.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | henrik molina |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; |
ModificationList | acetylated residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-08-30 01:21:56 | ID requested | |
⏵ 1 | 2019-11-18 01:16:55 | announced | |
Publication List
Gupta MP, Tandalam S, Ostrager S, Lever AS, Fung AR, Hurley DD, Alegre GB, Espinal JE, Remmel HL, Mukherjee S, Levine BM, Robins RP, Molina H, Dill BD, Kenific CM, Tuschl T, Lyden D, D'Amico DJ, Pena JTG, Non-reversible tissue fixation retains extracellular vesicles for in situ imaging. Nat Methods, 16(12):1269-1273(2019) [pubmed] |
Keyword List
submitter keyword: imaging extracellular vesicles fixation tissue |
Contact List
Pena, John T.G. |
contact affiliation | Weill Cornell Medicine |
contact email | jpena65@gmail.com |
lab head | |
henrik molina |
contact affiliation | THE ROCKEFELLER UNIVERSITY |
contact email | henrik.molina@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD015234 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015234
- Label: PRIDE project
- Name: Non-reversible tissue fixation by EDC carbodiimide allows for imaging extracellular vesicles in situ