PXD015233

PXD015233 is an original dataset announced via ProteomeXchange.

Title	Lysine fatty-acyl H3 peptide pulldown with HeLa SILAC nuclear extract
Description	Histone H3 peptides with acyl modification on either K9 or K27 were used for peptide pull-down with stable isotope (light: K0R0, heavy: K8R10) labeled Hela nuclear extract. The acyl modifications are: acetyl (Ac), propionyl (Pro), Butanoyl (But), crotonyl (Cr), 3-hydroxy butanoyl (bHB), succinyl (Su), Pentanoyl (Vl), Hexanoyl (Xho) and Myristoyl (Myr). Each modified peptide is compared with an unmodified peptide with the same amino acid sequence. Both forward (unmodified-light, acyl-heavy) and reverse (unmodified-heavy, acyl-light) labeling and pull-down experiments were performed.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:57:11.885.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Coan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-30 01:21:31	ID requested
1	2019-10-02 03:51:14	announced
2	2019-10-07 02:10:05	announced	2019-10-07: Updated publication reference for PubMed record(s): 31578417.
⏵ 3	2024-10-22 04:57:12	announced	2024-10-22: Updated project metadata.

10.1038/s41598-019-50817-6;

Li TM, Coan JP, Krajewski K, Zhang L, Elias JE, Strahl BD, Gozani O, Chua KF, Binding to medium and long chain fatty acyls is a common property of HEAT and ARM repeat modules. Sci Rep, 9(1):14226(2019) [pubmed]

submitter keyword: Orbitrap Elite,Homo sapiens

Katrin Chua
contact affiliation	1Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
contact email	kfchua@stanford.edu
lab head
John Coan
contact affiliation	Stanford University
contact email	jcoan@stanford.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD015233

PRIDE project URI

• PRIDE
  1. PXD015233
     1. Label: PRIDE project
     2. Name: Lysine fatty-acyl H3 peptide pulldown with HeLa SILAC nuclear extract