PXD015231

PXD015231 is an original dataset announced via ProteomeXchange.

Title	Defining the interactome of CHD4 in fusion-positive rhabdomyosarcoma
Description	The goal of this study was to explore in detail how the chromatin remodeler and NuRD subunit CHD4 controls the oncogenic signature of the tumor driver and fusion protein PAX3-FOXO1 in fusion-positive rhabdomyosarcoma. To this aim, we defined the interactome of CHD4 by LC-MS, identified its location in the genome by ChIP-seq, assessed its influence on DNA accessibility by DNase I hypersensitivity assays, and determined its target genes by RNA-seq.
HostingRepository	PRIDE
AnnounceDate	2020-08-11
AnnouncementXML	Submission_2020-08-22_16:45:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fabian Frommelt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-30 01:04:32	ID requested
1	2020-08-04 23:20:37	announced
⏵ 2	2020-08-22 16:45:54	announced	2020-08-23: Updated publication reference for PubMed record(s): 32744500.

Marques JG, Gryder BE, Pavlovic B, Chung Y, Ngo QA, Frommelt F, Gstaiger M, Song Y, Benischke K, Laubscher D, Wachtel M, Khan J, Sch, ä, fer BW, NuRD subunit CHD4 regulates super-enhancer accessibility in rhabdomyosarcoma and represents a general tumor dependency. Elife, 9():(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: CHD4, alveolar rhabdomyosarcoma, NuRD, RH4

Beat W. Schäfer
contact affiliation	Department of Oncology, Children’s Hospital Zurich, Steinwiesstrasse 75, 8032 Zurich, Switzerland
contact email	beat.schaefer@kispi.uzh.ch
lab head
Fabian Frommelt
contact affiliation	ETH Zurich
contact email	frommelt@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD015231

PRIDE project URI

• PRIDE
  1. PXD015231
     1. Label: PRIDE project
     2. Name: Defining the interactome of CHD4 in fusion-positive rhabdomyosarcoma