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PXD015220

PXD015220 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLabel-free quantitative proteomics versus antibody-based assays to measure neutrophil derived enzymes in saliva
DescriptionDiscovery-based quantitative proteomics identified dozens of novel protein candidates in saliva, yet none are clinically qualified. Mass spectrometry (MS) has been recognized as newer 'gold standard' for the identification and analysis of individual proteins in complex biological samples due to higher sensitivity, specificity and independence of antibody availability. However, whether MS can replace immunoassays for salivary biomarker validation has not been thoroughly studied. Therefore, we aimed to test the potential of label-free quantitative (LFQ) proteomics in comparison to conventional antibody-based methods to quantify ‘candidate biomarkers’ in saliva during experimentally-induced oral inflammation development in humans. Whole unstimulated saliva, collected at five time points, was analyzed by LFQ shotgun proteomics. Salivary levels of ‘candidate biomarkers’ were also assessed by antibody-based immunoassays such as ELISA and time-resolved immunofluorometric assay (IFMA).
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:58:10.437.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKai Bao
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmethionine oxidation with neutral loss of 64 Da; N-acylated residue; iodoacetamide derivatized residue
InstrumentLCQ Classic; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-29 05:53:22ID requested
12020-01-10 01:59:53announced
22024-10-22 04:58:15announced2024-10-22: Updated project metadata.
Publication List
10.1002/prca.201900050;
Silbereisen A, Alassiri S, Bao K, Grossmann J, Nanni P, Fernandez C, Tervahartiala T, Nascimento GG, Belibasakis GN, Heikkinen AM, Lopez R, Sorsa T, Bostanci N, Label-Free Quantitative Proteomics versus Antibody-Based Assays to Measure Neutrophil-Derived Enzymes in Saliva. Proteomics Clin Appl, 14(3):e1900050(2020) [pubmed]
Keyword List
submitter keyword: saliva,Huamn experimental gingivitis
Contact List
Nagihan Bostanci
contact affiliationProfessor, Department of Dental Medicine (DENTMED), OF, Karolinska institutet
contact emailnagihan.bostanci@ki.se
lab head
Kai Bao
contact affiliationpostdoctoral researcher
contact emailkai.bao@ki.se
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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