PXD015220

PXD015220 is an original dataset announced via ProteomeXchange.

Title	Label-free quantitative proteomics versus antibody-based assays to measure neutrophil derived enzymes in saliva
Description	Discovery-based quantitative proteomics identified dozens of novel protein candidates in saliva, yet none are clinically qualified. Mass spectrometry (MS) has been recognized as newer 'gold standard' for the identification and analysis of individual proteins in complex biological samples due to higher sensitivity, specificity and independence of antibody availability. However, whether MS can replace immunoassays for salivary biomarker validation has not been thoroughly studied. Therefore, we aimed to test the potential of label-free quantitative (LFQ) proteomics in comparison to conventional antibody-based methods to quantify ‘candidate biomarkers’ in saliva during experimentally-induced oral inflammation development in humans. Whole unstimulated saliva, collected at five time points, was analyzed by LFQ shotgun proteomics. Salivary levels of ‘candidate biomarkers’ were also assessed by antibody-based immunoassays such as ELISA and time-resolved immunofluorometric assay (IFMA).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:58:10.437.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kai Bao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	methionine oxidation with neutral loss of 64 Da; N-acylated residue; iodoacetamide derivatized residue
Instrument	LCQ Classic; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-29 05:53:22	ID requested
1	2020-01-10 01:59:53	announced
⏵ 2	2024-10-22 04:58:15	announced	2024-10-22: Updated project metadata.

10.1002/prca.201900050;

Silbereisen A, Alassiri S, Bao K, Grossmann J, Nanni P, Fernandez C, Tervahartiala T, Nascimento GG, Belibasakis GN, Heikkinen AM, Lopez R, Sorsa T, Bostanci N, Label-Free Quantitative Proteomics versus Antibody-Based Assays to Measure Neutrophil-Derived Enzymes in Saliva. Proteomics Clin Appl, 14(3):e1900050(2020) [pubmed]

submitter keyword: saliva,Huamn experimental gingivitis

Nagihan Bostanci
contact affiliation	Professor, Department of Dental Medicine (DENTMED), OF, Karolinska institutet
contact email	nagihan.bostanci@ki.se
lab head
Kai Bao
contact affiliation	postdoctoral researcher
contact email	kai.bao@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD015220

PRIDE project URI

• PRIDE
  1. PXD015220
     1. Label: PRIDE project
     2. Name: Label-free quantitative proteomics versus antibody-based assays to measure neutrophil derived enzymes in saliva