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PXD015212

PXD015212 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCo-immunoprecipitation of FAMA-interacting proteins in Arabidopsis thaliana seedlings
DescriptionIdentifying interaction partners and protein complex compositions for transcription factors (TFs) can produce valuable information on the mechanisms by which they regulate gene expression or are themselves regulated by signaling pathways in the cell. FAMA is a TF that is specifically expressed in the last stages of stomatal guard cell development in the epidermis of young leaves and other aerial tissues of higher plants. It is a master regulator of guard cell development and promotes terminal differentiation of the guard cell precursor by both activating and repressing hundreds of genes. How this is achieved mechanistically is still unclear. We therefore isolated putative FAMA interaction partners from young Arabidopsis seedlings expressing FAMA-CFP under the FAMA promoter using proteasome inhibitor treatment and formaldehyde crosslinking, followed by a classical co-immunoprecipitation mass spectrometry approach. Plants expressing nuclear GFP under a stomatal lineage specific promoter were used as controls. In two independent experiments with different crosslinking and immunoprecipitation conditions, we found ICE1, a known heterodimerization partner of FAMA, to be enriched in FAMA-CFP versus control samples. Other proteins with known transcriptional regulator or co-regulator function were not identified, presumably due to the low abundance of the bait protein. The interaction of FAMA with BRXL2, another regulator of guard cell development, which was detected in one experiment, is likely an artifact since BRXL2 does not localize to the nucleus under normal conditions.
HostingRepositoryPRIDE
AnnounceDate2019-09-26
AnnouncementXMLSubmission_2019-09-26_08:25:04.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterShouling Xu
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-29 04:22:52ID requested
12019-09-26 08:25:05announced
Publication List
Mair A, Xu SL, Branon TC, Ting AY, Bergmann DC, enabled by TurboID. Elife, 8():(2019) [pubmed]
Keyword List
submitter keyword: Plant, seedling, Arabidopsis thaliana, bHLH transcription factor, FAMA, stomatal guard cell, interaction partners, co-immunoprecipitation, LC-MS/MS
Contact List
Shouling Xu
contact affiliationCarnegie Mass Spectometry facility Carnegie Science at Stanford
contact emailsxu@ciw.edu
lab head
Shouling Xu
contact affiliationCarnegie Institution at Stanford
contact emailsxu@ciw.edu
dataset submitter
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