PXD015199

PXD015199 is an original dataset announced via ProteomeXchange.

Title	Identification of full length, splicing and cleavage products of SufB protein of SUF-complex of Mycobacterium tuberculosis
Description	SufB, an Fe-S cluster assembly protein is critical for mycobacterial survival and virulence; but as a protein still at its nascent stage. SufB is interrupted by an intein sequence, which excises itself out from the full length protein post-translationally. Intein splicing is crucial for the activation of SufB, where intein(I) is excised and final functional product is generated by the ligation of flanking regions, known as Extein or Ligated extein(LE). Functional SufB is coordinating with other Suf proteins of SUF complex to generate Fe-S cluster. These are important for the central metabolism, gene expression, DNA repair etc. During the process of splicing, SufB has a tendency to generate off pathway products as N-cleavage (NC) and N-Extein (NE). In this project we have confirmed the identities of Mycobacterium tuberculosis full length SufB protein (precursor/p), SufB Intein (i), SufB Extein (ligated Extein/le), N-terminal cleavage product (nc) and N-extein (ne) by MALDI TOF/TOF mass spectrometry via gel based proteomics method .
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_09:46:45.804.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SASMITA NAYAK
SpeciesList	scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationList	iodoacetamide derivatized residue
Instrument	5800 TOF/TOF

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-28 07:10:01	ID requested
⏵ 1	2022-02-15 09:46:46	announced

Nanda A, Nasker SS, Kushwaha AK, Ojha DK, Dearden AK, Nayak SK, Nayak S, SufB. Front Bioeng Biotechnol, 9():773303(2021) [pubmed]

submitter keyword: SufB, splicing, cleavage products, Mycobacterium tuberculosis

Dr Sasmita Nayak
contact affiliation	KIIT Deemed to be University
contact email	sasmita.n@kiitbiotech.ac.in
lab head
SASMITA NAYAK
contact affiliation	KIIT University
contact email	sasmita.n@kiitbiotech.ac.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD015199

PRIDE project URI

• PRIDE
  1. PXD015199
     1. Label: PRIDE project
     2. Name: Identification of full length, splicing and cleavage products of SufB protein of SUF-complex of Mycobacterium tuberculosis