PXD015192

PXD015192 is an original dataset announced via ProteomeXchange.

Title	How to cope with heavy metal ions: Insights into cellular and molecular stress response in Halobacterium salinarum R1 planktonic cells and biofilms
Description	Halobacterium salinarum R1 is an extremely halophilic archaeon capable of adhesion and biofilm formation. We have recently shown that living in biofilms facilitates higher cell survival under heavy metal ion stress in this species, while specific rearrangements of the biofilm architecture were observed upon Ni2+ and Cu2+ exposition, respectively. In this study, quantitative analyses were performed by SWATH-LC-MS/MS to determine the respective proteomes under the influences of Ni2+ and Cu2+ in planktonic and biofilm cells. Quantitative data for 1180 proteins were gained, corresponding to 46% of the predicted proteome. In planktonic cells, 234 proteins showed significant abundance changes after metal ion treatment, of which 47% occurred combined in Cu2+ and Ni2+ treated samples. Regarding biofilms, significant changes were detected for 52 proteins. Only three proteins changed under both conditions, suggesting metal-specific stress responses in biofilms.
HostingRepository	PRIDE
AnnounceDate	2020-05-26
AnnouncementXML	Submission_2020-05-26_15:41:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christof Lenz
SpeciesList	scientific name: Halobacterium salinarum; NCBI TaxID: 2242;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-28 00:47:02	ID requested
⏵ 1	2020-05-26 15:41:37	announced

V, ö, lkel S, Hein S, Benker N, Pfeifer F, Lenz C, Losensky G, R1 Planktonic and Biofilm Cells. Front Microbiol, 10():3056(2019) [pubmed]

curator keyword: Biological

submitter keyword: label-free quantitation, SWATH-MS, archaea, Halobacterium, stress response

Christof Lenz
contact affiliation	Max Planck Institute for Biophysical Chemistry
contact email	christof.lenz@mpibpc.mpg.de
lab head
Christof Lenz
contact affiliation	Max Planck Institute for Biophysical Chemistry
contact email	christof.lenz@mpibpc.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015192
     1. Label: PRIDE project
     2. Name: How to cope with heavy metal ions: Insights into cellular and molecular stress response in Halobacterium salinarum R1 planktonic cells and biofilms