PXD015185

PXD015185 is an original dataset announced via ProteomeXchange.

Title	Rapid acetylation of yeast Hsp70 mediates the cellular response to heat stress
Description	Hsp70 is a highly conserved molecular chaperone critical for the folding of new and denatured proteins. While traditional models state that cells respond to stress by upregulating inducible HSPs, this response is relatively slow and is limited by transcriptional and translational machinery. Recent studies have identified a number of post-translational modifications (PTMs) on Hsp70 that act to fine-tune its function. We utilized mass spectrometry to determine whether yeast Hsp70 (Ssa1) is differentially modified upon heat shock. We uncovered four lysine residues on Ssa1, K86, K185, K354 and K562 that are deacetylated in response to heat shock. Mutation of these sites cause a substantial remodeling of the Hsp70 interaction network of co-chaperone partners and client proteins while preserving essential chaperone function. Acetylation/deacetylation at these residues alter expression of other heat-shock induced chaperones as well as directly influencing Hsf1 activity. Taken together our data suggest that cells may have the ability to respond to heat stress quickly though Hsp70 deacetylation, followed by a slower, more traditional transcriptional response.
HostingRepository	PRIDE
AnnounceDate	2020-02-24
AnnouncementXML	Submission_2020-02-23_23:55:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Donald Wolfgeher
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; acetylated residue
Instrument	LTQ; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-27 08:32:46	ID requested
⏵ 1	2020-02-23 23:55:23	announced

Xu L, Nitika, Hasin N, Cuskelly DD, Wolfgeher D, Doyle S, Moynagh P, Perrett S, Jones GW, Truman AW, Rapid deacetylation of yeast Hsp70 mediates the cellular response to heat stress. Sci Rep, 9(1):16260(2019) [pubmed]

submitter keyword: Yeast,Ssa1, Acetylation, Mass Spectrometry, PTMs, Q-Exactive, Heat Stress

Dr. Andrew W. Truman
contact affiliation	Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte, USA.
contact email	A.Truman@uncc.edu
lab head
Donald Wolfgeher
contact affiliation	University of Chicago
contact email	donw@uchicago.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015185
     1. Label: PRIDE project
     2. Name: Rapid acetylation of yeast Hsp70 mediates the cellular response to heat stress