PXD015184
PXD015184 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | COMPARATIVE OMICS ANALYSIS OF HISTORICAL AND RECENT ISOLATES OF BORDETELLA PERTUSSIS |
| Description | Despite high vaccination coverage, pertussis is on the rise in many countries including Czech Republic. To better understand B. pertussis resurgence we compared the changes in genome structures between Czech vaccine and circulating strains and subsequently, we determined how these changes translated into global transcriptomic and proteomic profiles. The whole-genome sequencing revealed that both historical and recent isolates of B. pertussis display substantial variation in genome organization and cluster separately. The RNA-seq and LC-MS/MS analyses indicate that these variations translated into discretely separated transcriptomic and proteomic profiles. Compared to vaccine strains, recent isolates displayed increased expression of flagellar genes and decreased expression of polysaccharide capsule operon. Czech strains (Bp46, K10, Bp155, Bp318 and Bp6242)exhibited increased expression of T3SS and sulphate metabolism genes when compared to Tohama I. In spite of 50 years of vaccination the Czech vaccine strains (VS67, VS393 and VS401) differ from recent isolates to a lesser extent than from another vaccine strain Tohama I. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_05:26:55.122.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Denisa Petrackova |
| SpeciesList | scientific name: Bordetella pertussis; NCBI TaxID: 520; scientific name: Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251); NCBI TaxID: 257313; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2019-08-27 07:51:08 | ID requested | |
| 1 | 2021-09-08 15:25:10 | announced | |
| ⏵ 2 | 2024-10-22 05:26:59 | announced | 2024-10-22: Updated project metadata. |
Publication List
| Dienstbier A, Amman F, Petr, á, č, kov, á D, Š, tipl D, Č, apek J, Zavadilov, á J, Fabi, á, nov, á K, Dr, ž, m, í, š, ek J, Kumar D, Wildung M, Pouchnik D, Ve, č, erek B, Comparative Omics Analysis of Historic and Recent Isolates of Bordetella pertussis and Effects of Genome Rearrangements on Evolution. Emerg Infect Dis, 27(1):57-68(2021) [pubmed] |
| 10.3201/eid2701.191541; |
Keyword List
| submitter keyword: adaptation, IS elements, genomic rearrangement, omics analysis,Bordetella pertussis |
Contact List
| Branislav Večerek | |
|---|---|
| contact affiliation | Academy of Sciences of the Czech Republic Institute of Microbiology, v.v.i. Prague, Czech Republic |
| contact email | vecerek@biomed.cas.cz |
| lab head | |
| Denisa Petrackova | |
| contact affiliation | Institute of Microbiology of the CAS, v. v. i. |
| contact email | petrack@biomed.cas.cz |
| dataset submitter | |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD015184 |
| PRIDE project URI |
Repository Record List
[ + ]
to receive all new ProteomeXchange dataset release announcements!
