PXD015183

PXD015183 is an original dataset announced via ProteomeXchange.

Title	SUMOylation regulates chromatin protein network and accessibility at glucocorticoid receptor-binding sites
Description	Glucocorticoid receptor (GR) is an essential transcription factor (TF), controlling metabolism, development and immune responses. SUMOylation regulates chromatin occupancy and target gene expression of GR in a locus-selective manner, but the mechanism of regulation has remained elusive. Here, we show using selective isolation of chromatin-associated proteins that the protein network around chromatin-bound GR is affected by SUMOylation, with several nuclear receptor coregulators and chromatin modifiers being more avidly associated with SUMOylation-deficient than SUMOylation competent GR. This difference is reflected in our chromatin accessibility and gene expression data, showing that the SUMOylation-deficient GR is more potent in opening chromatin at glucocorticoid-regulated enhancers and inducing expression of their target loci. Our results thus show that SUMOylation determines GR specificity by regulating the chromatin protein network and accessibility at GR-driven enhancers. We speculate that a similar mechanism is utilized by many other SUMOylated TFs.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:26:34.908.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gianluca Sigismondo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-27 07:45:51	ID requested
1	2021-09-08 13:49:51	announced
⏵ 2	2024-10-22 05:26:39	announced	2024-10-22: Updated project metadata.

Paakinaho V, Lempi, ä, inen JK, Sigismondo G, Niskanen EA, Malinen M, J, ä, ä, skel, ä, inen T, Varjosalo M, Krijgsveld J, Palvimo JJ, SUMOylation regulates the protein network and chromatin accessibility at glucocorticoid receptor-binding sites. Nucleic Acids Res, 49(4):1951-1971(2021) [pubmed]

10.1093/nar/gkab032;

submitter keyword: glucocorticoid receptor, proteomics, chromatin regulation,SUMOylation, transcription factors

Jorma J. Palvimo
contact affiliation	Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland
contact email	jorma.palvimo@uef.fi
lab head
Gianluca Sigismondo
contact affiliation	DKFZ
contact email	gianluca.sigismondo@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD015183

PRIDE project URI

• PRIDE
  1. PXD015183
     1. Label: PRIDE project
     2. Name: SUMOylation regulates chromatin protein network and accessibility at glucocorticoid receptor-binding sites