PXD015175

PXD015175 is an original dataset announced via ProteomeXchange.

Title	Filter aided, single tip (FAST) method for high throughput, ultrasensitive proteomics sample preparation reveals proteome asymmetry in Xenopus embryos
Description	We report a filter aided, single tip (FAST) method for ultrasensitive proteomic sample preparation. To minimize sample loss and contamination, the method reduces the surface area of the filter to ~0.1 mm2, the total volume of reagents to < 10 μL, and the number of sample transfer steps to two. 25,883 unique peptides and 3,069 protein groups were identified from 1,000 MCF-7 cells (~100 ng protein content). Single blastomeres from Xenopus laevis embryos at the 50-cell stage (~200 ng yolk free protein/blastomere) generated 20,943 unique peptides and 2,597 protein groups; the proteomic profile clearly differentiated left and right blastomeres, and provides strong support for models in which this asymmetry is established early in the embryo.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:22.528.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhenbin Zhang
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-27 06:41:56	ID requested
1	2022-07-12 07:58:30	announced
⏵ 2	2023-11-14 08:50:23	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: single blastomere,sample preparation, ultrasensitive proteomics

Zhenbin Zhang
contact affiliation	University of Notre Dame
contact email	zzhang9@nd.edu
lab head
Zhenbin Zhang
contact affiliation	Ningbo University
contact email	zzhang9@nd.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015175
     1. Label: PRIDE project
     2. Name: Filter aided, single tip (FAST) method for high throughput, ultrasensitive proteomics sample preparation reveals proteome asymmetry in Xenopus embryos