PXD015165

PXD015165 is an original dataset announced via ProteomeXchange.

Title	Next-generation diagnostic biomarkers for vivax malaria
Description	The global impact of Plasmodium vivax has been largely underestimated for several decades due to lower mortality rates compared to P. falciparum. However, in recent times, the parasite has become a serious threat to public health due to its ability to cause severe malaria with fatal outcomes. Its unique biology makes it resilient to control measures and poses a challenge to available diagnostic methods. Diagnosis by RDTs is further restricted due to inadequate P. vivax specific antigens for species identification. Therefore, there is an urgent need to develop tests that employ antigens unique to the parasite. This study represents the first in-depth proteomics analysis of human plasma and parasite isolates to identify P. vivax protein biomarkers that can be tested for use in RDTs while developing diagnostics for malaria. Here we report 39 P. vivax proteins in human plasma and 103 highly expressed P. vivax proteins from parasite isolates with high confidence. Interestingly, five proteins, found to be unique to P. vivax were detected in both sources, representing the best candidates for evaluation as diagnostic markers. Moreover, targeted proteomics assays were used to validate some of these proteins. This study represents the first step in the development of new diagnostic assays for P. vivax malaria.
HostingRepository	PRIDE
AnnounceDate	2020-01-30
AnnouncementXML	Submission_2020-01-30_06:43:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shalini Aggarwal
SpeciesList	scientific name: Plasmodium vivax; NCBI TaxID: 5855;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-27 05:47:05	ID requested
⏵ 1	2020-01-30 06:43:15	announced

Dataset with its publication pending

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Malaria Host-Pathogen Interaction Center (MaHPIC)

submitter keyword: Malaria, diagnosis, biomarker candidates, Multiple Reaction Monitoring, parasite, Q Exactive Orbitrap, mass spectrometry

Prof. Sanjeeva Srivastava
contact affiliation	Indian Institute of Technology Bombay
contact email	sanjeeva@iitb.ac.in
lab head
Shalini Aggarwal
contact affiliation	IIT Bombay
contact email	shalini.a@iitb.ac.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015165
     1. Label: PRIDE project
     2. Name: Next-generation diagnostic biomarkers for vivax malaria