PXD015143
PXD015143 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Common Repository of FBS Proteins (cRFP) to be added to a search database for mass spectrometric analysis of cell secretome |
Description | We propose to use cRFP (common Repository of FBS Proteins) in the MS (mass spectrometry) raw data search of cell secretome. cRFP is a small supplementary sequence list of high abundant fetal bovine serum proteins added to the reference database in use. The aim behind using cRFP is to prevent the contaminant FBS proteins from being misidentified as other proteins in the reference database, just as we would use cRAP (common Repository of Adventitious Proteins) to prevent contaminant proteins present either by accident or through unavoidable contacts from being misidentified as other proteins. We expect it to be widely used in experiments where the proteins are obtained from serum-free media after thorough washing of the cells, or from a complex media such as SILAC, or from extracellular vesicles directly. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:57:27.239.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yumi Kwon |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-08-23 06:04:41 | ID requested | |
1 | 2019-09-04 04:16:23 | announced | |
⏵ 2 | 2024-10-22 04:57:35 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1021/acs.jproteome.9b00475; |
Shin J, Kwon Y, Lee S, Na S, Hong EY, Ju S, Jung HG, Kaushal P, Shin S, Back JH, Choi SY, Kim EH, Lee SJ, Park YE, Ahn HS, Ahn Y, Kabir MH, Park SJ, Yang WS, Yeom J, Bang OY, Ha CW, Lee JW, Kang UB, Kim HJ, Park KS, Lee JE, Lee JE, Kim JY, Kim KP, Kim Y, Hirano H, Yi EC, Cho JY, Paek E, Lee C, Common Repository of FBS Proteins (cRFP) To Be Added to a Search Database for Mass Spectrometric Analysis of Cell Secretome. J Proteome Res, 18(10):3800-3806(2019) [pubmed] |
Keyword List
submitter keyword: common repository of FBS proteins, database search,Secretome |
Contact List
Cheolju Lee | |
---|---|
contact affiliation | Center for Theragnosis, Korea Institute of Science and Technology, Seoul, Korea |
contact email | clee270@kist.re.kr |
lab head | |
Yumi Kwon | |
contact affiliation | Korea Institute of Science and Technology |
contact email | ymkwon@kist.re.kr |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD015143 |
PRIDE project URI |
Repository Record List
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