PXD015143

PXD015143 is an original dataset announced via ProteomeXchange.

Title	Common Repository of FBS Proteins (cRFP) to be added to a search database for mass spectrometric analysis of cell secretome
Description	We propose to use cRFP (common Repository of FBS Proteins) in the MS (mass spectrometry) raw data search of cell secretome. cRFP is a small supplementary sequence list of high abundant fetal bovine serum proteins added to the reference database in use. The aim behind using cRFP is to prevent the contaminant FBS proteins from being misidentified as other proteins in the reference database, just as we would use cRAP (common Repository of Adventitious Proteins) to prevent contaminant proteins present either by accident or through unavoidable contacts from being misidentified as other proteins. We expect it to be widely used in experiments where the proteins are obtained from serum-free media after thorough washing of the cells, or from a complex media such as SILAC, or from extracellular vesicles directly.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:57:27.239.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yumi Kwon
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-23 06:04:41	ID requested
1	2019-09-04 04:16:23	announced
⏵ 2	2024-10-22 04:57:35	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.9b00475;

Shin J, Kwon Y, Lee S, Na S, Hong EY, Ju S, Jung HG, Kaushal P, Shin S, Back JH, Choi SY, Kim EH, Lee SJ, Park YE, Ahn HS, Ahn Y, Kabir MH, Park SJ, Yang WS, Yeom J, Bang OY, Ha CW, Lee JW, Kang UB, Kim HJ, Park KS, Lee JE, Lee JE, Kim JY, Kim KP, Kim Y, Hirano H, Yi EC, Cho JY, Paek E, Lee C, Common Repository of FBS Proteins (cRFP) To Be Added to a Search Database for Mass Spectrometric Analysis of Cell Secretome. J Proteome Res, 18(10):3800-3806(2019) [pubmed]

submitter keyword: common repository of FBS proteins, database search,Secretome

Cheolju Lee
contact affiliation	Center for Theragnosis, Korea Institute of Science and Technology, Seoul, Korea
contact email	clee270@kist.re.kr
lab head
Yumi Kwon
contact affiliation	Korea Institute of Science and Technology
contact email	ymkwon@kist.re.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD015143

PRIDE project URI

• PRIDE
  1. PXD015143
     1. Label: PRIDE project
     2. Name: Common Repository of FBS Proteins (cRFP) to be added to a search database for mass spectrometric analysis of cell secretome