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PXD015143

PXD015143 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCommon Repository of FBS Proteins (cRFP) to be added to a search database for mass spectrometric analysis of cell secretome
DescriptionWe propose to use cRFP (common Repository of FBS Proteins) in the MS (mass spectrometry) raw data search of cell secretome. cRFP is a small supplementary sequence list of high abundant fetal bovine serum proteins added to the reference database in use. The aim behind using cRFP is to prevent the contaminant FBS proteins from being misidentified as other proteins in the reference database, just as we would use cRAP (common Repository of Adventitious Proteins) to prevent contaminant proteins present either by accident or through unavoidable contacts from being misidentified as other proteins. We expect it to be widely used in experiments where the proteins are obtained from serum-free media after thorough washing of the cells, or from a complex media such as SILAC, or from extracellular vesicles directly.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:57:27.239.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYumi Kwon
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-23 06:04:41ID requested
12019-09-04 04:16:23announced
22024-10-22 04:57:35announced2024-10-22: Updated project metadata.
Publication List
10.1021/acs.jproteome.9b00475;
Shin J, Kwon Y, Lee S, Na S, Hong EY, Ju S, Jung HG, Kaushal P, Shin S, Back JH, Choi SY, Kim EH, Lee SJ, Park YE, Ahn HS, Ahn Y, Kabir MH, Park SJ, Yang WS, Yeom J, Bang OY, Ha CW, Lee JW, Kang UB, Kim HJ, Park KS, Lee JE, Lee JE, Kim JY, Kim KP, Kim Y, Hirano H, Yi EC, Cho JY, Paek E, Lee C, Common Repository of FBS Proteins (cRFP) To Be Added to a Search Database for Mass Spectrometric Analysis of Cell Secretome. J Proteome Res, 18(10):3800-3806(2019) [pubmed]
Keyword List
submitter keyword: common repository of FBS proteins, database search,Secretome
Contact List
Cheolju Lee
contact affiliationCenter for Theragnosis, Korea Institute of Science and Technology, Seoul, Korea
contact emailclee270@kist.re.kr
lab head
Yumi Kwon
contact affiliationKorea Institute of Science and Technology
contact emailymkwon@kist.re.kr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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