PXD015139

PXD015139 is an original dataset announced via ProteomeXchange.

Title	Unbalanced roles of fungal aggressiveness and host genetic background in the establishment of the Fusarium head blight in bread wheat
Description	Fungal effectors are key determinants of disease development, promoting pathogen growth and spreading in plant tissues. In the wheat/Fusarium graminearum (Fg) interaction determining the Fusarium Head Blight disease (FHB), the molecular bases of Fg aggressiveness remain widely unknown. The aim of this work is to provide a better understanding of Fusarium graminearum aggressiveness determinism during its interaction with bread wheat. Phenotyping in controlled conditions of three fungal strains was carried out on three wheat cultivars contrasted by their susceptibility to FHB. Qualitative and quantitative characterizations of the three strain's whole proteomes expressed in planta have been performed with special interest on effectors and proteins coded by Fg aggressiveness-related genes. Considering all fusaried wheat samples, this experimentation identified 615 unique Fg proteins, including 583 identified in all samples. Furthermore, 71 putative Fg effectors were found in the identified proteins. Among all the fungal proteins quantified, nearly 76% showed significant abundance differences between the Fg strains. Two-way ANOVA were computed to evaluate the contribution of the "Strain" and "Cultivar" factors on the Fg protein abundances. Abundances that were deemed significant to each factor enabled a categorization of the proteins according to the factor(s) that drive(s) the abundance differences. This included: (i) Fg proteins whose abundance differences were specifically explained by the genetic background of the strain (Strain factor, Strain_effect proteins), (ii) Fg proteins whose abundance differences were only explained by the genetic background of the host plant (Cultivar factor, Cultivar_effect proteins), (iii) Fg proteins whose abundance differences were explained by both factors (Strain+Cultivar proteins), and (iv) Fg proteins whose abundance differences observed between strains were dependent on the host cultivar (Strain×Cultivar proteins). Considering each factor individually, a total of 139 Strain_effect proteins, 82 Cultivar_effect proteins, 117 Strain+Cultivar proteins and 2 Strain×Cultivar proteins were identified.
HostingRepository	PRIDE
AnnounceDate	2020-01-13
AnnouncementXML	Submission_2020-01-17_06:14:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ludovic Bonhomme
SpeciesList	scientific name: Fusarium graminearum; NCBI TaxID: 5518; scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-23 06:02:54	ID requested
1	2020-01-13 04:50:34	announced
⏵ 2	2020-01-17 06:14:09	announced	2020-01-17: Updated publication reference for PubMed record(s): 31921038.

Fabre F, Bormann J, Urbach S, Roche S, Langin T, Bonhomme L, Unbalanced Roles of Fungal Aggressiveness and Host Cultivars in the Establishment of the Fusarium Head Blight in Bread Wheat. Front Microbiol, 10():2857(2019) [pubmed]

submitter keyword: Wheat, Fusarium graminearum, Fungal aggressiveness, Fusarium Head Blight, proteins

Thierry Langin
contact affiliation	UMR 1095 INRA / UCA Génétique, Diversité et Ecophysiologie des Céréales, Clermont-Ferrand, France
contact email	thierry.langin@inra.fr
lab head
Ludovic Bonhomme
contact affiliation	UMR UCA/INRA 1095
contact email	ludovic.bonhomme@inra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD015139

PRIDE project URI

• PRIDE
  1. PXD015139
     1. Label: PRIDE project
     2. Name: Unbalanced roles of fungal aggressiveness and host genetic background in the establishment of the Fusarium head blight in bread wheat