PXD015138

PXD015138 is an original dataset announced via ProteomeXchange.

Title	Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation
Description	Induction of DNA double-strand breaks (DSBs) in ribosomal DNA (rDNA) repeats is associated with ATM-dependent repression of ribosomal RNA synthesis and large-scale reorganization of nucleolar architecture, but the signaling events that regulate these responses are largely elusive. Here we show that the nucleolar response to rDNA breaks is dependent on both ATM and ATR activity. We further demonstrate that ATM- and NBS1-dependent recruitment of TOPBP1 in the nucleoli is required for inhibition of ribosomal RNA synthesis and nucleolar segregation in response to rDNA breaks. Mechanistically, TOPBP1 recruitment is mediated by phosphorylation-dependent interactions between three of its BRCT domains and conserved phosphorylated Ser/Thr residues at the C-terminus of the nucleolar phosphoprotein Treacle. Our data thus reveal an important cooperation between TOPBP1 and Treacle in the signaling cascade that triggers transcriptional inhibition and nucleolar segregation in response to rDNA breaks.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:56:58.597.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sara Larsen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-23 06:02:38	ID requested
1	2019-12-06 06:37:30	announced
2	2020-01-17 06:30:47	announced	2020-01-17: Updated publication reference for PubMed record(s): 31913317.
⏵ 3	2024-10-22 04:57:03	announced	2024-10-22: Updated project metadata.

Mooser C, Symeonidou IE, Leimbacher PA, Ribeiro A, Shorrocks AK, Jungmichel S, Larsen SC, Knechtle K, Jasrotia A, Zurbriggen D, Jeanrenaud A, Leikauf C, Fink D, Nielsen ML, Blackford AN, Stucki M, Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation. Nat Commun, 11(1):123(2020) [pubmed]

10.1038/s41467-019-13981-x;

submitter keyword: DNA double.strand breaks,Q Exactive HF, treacle

Michael Lund Nielsen
contact affiliation	NNF Center for Protein Research, University of Copenhagen
contact email	michael.lund.nielsen@cpr.ku.dk
lab head
Sara Larsen
contact affiliation	NNF Center for Protein Research
contact email	sara.larsen@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD015138

PRIDE project URI

• PRIDE
  1. PXD015138
     1. Label: PRIDE project
     2. Name: Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation