PXD015137

PXD015137 is an original dataset announced via ProteomeXchange.

Title	hnRNP M and HSP 90α as Potential Biomarkers in Early Stage Cutaneous Head and Neck Melanoma Tissue
Description	Background: Timely diagnosis is important for successful treatment of cutaneous melanoma. Currently, Breslow tumor thickness and mitotic rate are used for malignant melanoma classification and prognosis, but these parameters can assess disease progression risk only to a certain degree. Therefore, there is a need for new melanoma protein biomarkers that would aid early and accurate diagnosis and prediction of their metastatic potential. Methodology and Findings: This retrospective case control study is based on proteomic profiling of formalin-fixed archival tissues of 31 early-stage head and neck cutaneous malignant melanoma samples using liquid chromatography / mass spectrometry. A melanoma proteomic profile was identified and protein expression levels were compared to the proteome profile of melanocytic naevi and correlated to established prognostic factors and disease-specific survival. In accordance with the American Joint Committee on Cancer guidelines, recursive partitioning multivariate analysis was used to identify potential biomarkers associated with metastatic potential of early-melanoma. Heterogeneous nuclear ribonucleoprotein M and heat shock protein 90 alpha were profiled as independent prognostic factors. Their elevated expression was clinically relevant for predicting an exceedingly high metastatic hazard ratio. These proteins were superior in estimating disease progression risk when compared to Breslow thickness and mitotic rate. Conclusions and Significance: Identification of biomarkers in early stage cutaneous head and neck melanoma is an important step towards predicting metastatic potential and prognosis of the disease. Clinical confirmation and further validation of the proteins identified in this study would provide a novel tool for identifying patients at risk for developing metastatic disease.
HostingRepository	PRIDE
AnnounceDate	2019-11-27
AnnouncementXML	Submission_2020-01-14_07:39:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ruđer Novak
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Discovery

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-23 06:02:18	ID requested
1	2019-11-27 04:29:49	announced
⏵ 2	2020-01-14 07:39:30	announced	2020-01-14: Updated publication reference for PubMed record(s): 31803364.

Ko, š, ec A, Novak R, Konjevoda P, Trkulja V, Bedekovi, ć V, Grgurevi, ć L, as potential predictors of disease-specific mortality in patients with early-stage cutaneous head and neck melanoma: A proteomics-based study. Oncotarget, 10(62):6713-6722(2019) [pubmed]

submitter keyword: melanoma

early stage

proteomics

tissue

biomarkers

hnRNP M

HSP 90α

Lovorka Grgurević
contact affiliation	Department for Proteomics, Center for Translational and Clinical Research, School of Medicine, University of Zagreb Department of Anatomy “Drago Perović”, School of Medicine, University of Zagreb
contact email	lovorka.grgurevic@mef.hr
lab head
Ruđer Novak
contact affiliation	School of Medicine, University of Zagreb
contact email	rudjer.novak@mef.hr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015137
     1. Label: PRIDE project
     2. Name: hnRNP M and HSP 90α as Potential Biomarkers in Early Stage Cutaneous Head and Neck Melanoma Tissue