PXD015134

PXD015134 is an original dataset announced via ProteomeXchange.

Title	Identification of SENP6 target proteins by endogenous SUMO2/3 IP-MS
Description	Cellular signaling by the ubiquitin-related SUMO pathway relies on coordinated conjugation and deconjugation events. SUMO-specific deconjugating enzymes counterbalance SUMOylation, but comprehensive insight into their substrate specificity and regulation is missing. By characterizing SENP6 we define an N-terminal multi-SIM domain as critical determinant in targeting SENP6 activity to SUMO chains. Using an integrated proteomic profiling approach we reveal a network of SENP6 functions at the crossroad of chromatin organization and DNA damage response (DDR). SENP6 acts as SUMO eraser on key organizers of telomeric and centromeric chromatin domains and determines the SUMOylation status and chromatin association of the cohesin complex. Importantly, SENP6 is part of the hPSO4/PRP19 complex that drives ATR-Chk1 activation during DDR. Intriguingly, SENP6 deficiency impairs chromatin-association of the ATR cofactor ATRIP thereby compromising the activation of Chk1 signaling in response to replicative stress and sensitizing cells to replicative stress-induced DNA damage. Collectively, we propose a general role of SENP6 in orchestrating chromatin dynamics and genome stability networks by balancing chromatin-residency of protein complexes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:58:50.067.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kathrin Schunck
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	sumoylated lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-22 06:39:55	ID requested
1	2019-11-20 07:06:58	announced
2	2019-11-27 06:53:29	announced	2019-11-27: Updated project metadata.
⏵ 3	2024-10-22 04:58:57	announced	2024-10-22: Updated project metadata.

10.1016/j.celrep.2019.08.106;

Wagner K, Kunz K, Piller T, Tascher G, H, ö, lper S, Stehmeier P, Keiten-Schmitz J, Schick M, Keller U, M, ü, ller S, The SUMO Isopeptidase SENP6 Functions as a Rheostat of Chromatin Residency in Genome Maintenance and Chromosome Dynamics. Cell Rep, 29(2):480-494.e5(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: SUMO, human, SENP, LC-MS/MS

Prof. Dr. Stefan Müller
contact affiliation	Institute of Biochemistry II • Goethe University Medical School University Hospital Building 75 • Theodor-Stern-Kai 7 • 60590 Frankfurt, Germany
contact email	ste.mueller@em.uni-frankfurt.de
lab head
Kathrin Schunck
contact affiliation	Goethe University, Medical School, Institute of Biochemistry II, Frankfurt am Main
contact email	K.Schunck@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD015134

PRIDE project URI

• PRIDE
  1. PXD015134
     1. Label: PRIDE project
     2. Name: Identification of SENP6 target proteins by endogenous SUMO2/3 IP-MS