PXD015106

PXD015106 is an original dataset announced via ProteomeXchange.

Title	Quorum sensing elicits functionally distinct subpopulations of infectious Legionella
Description	Clonal bacterial populations respond to unfavorable conditions with growth arrest and 20 persistence. Here we show that quorum sensing (QS) determines functionally distinct subpopulations of non-growing, virulent Legionella. QS increases the fraction of bacteria re- initiating growth in microcolonies, while it restricts the ratio of bacteria resuming growth in free- living protozoan predators. QS continues to govern the population heterogeneity throughout growth. Intracellular non-growers and growing bacteria are characterized by distinct proteomes 25 reflecting different stress and drug tolerance as well as alternative metabolic pathways. Strikingly, individual intracellular non-growers are persisters, which endure host killing mechanisms, produce virulence factors, form a replication-permissive compartment, and are highly infectious. Thus, QS underlies a bet-hedging strategy of non-growing intracellular bacterial pathogens to resume growth and to fine-tune virulence.
HostingRepository	PRIDE
AnnounceDate	2019-11-27
AnnouncementXML	Submission_2019-11-27_06:52:42.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015106
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Schmidt
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Legionella pneumophila subsp. pneumophila; NCBI TaxID: 91891;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-21 01:08:01	ID requested
1	2019-11-19 13:24:05	announced
2	2019-11-21 15:06:08	announced	2019-11-21: Updated publication reference for PubMed record(s): 31740681.
⏵ 3	2019-11-27 06:52:43	announced	2019-11-27: Updated project metadata.

Personnic N, Striednig B, Lezan E, Manske C, Welin A, Schmidt A, Hilbi H, Quorum sensing modulates the formation of virulent Legionella persisters within infected cells. Nat Commun, 10(1):5216(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: LFQ, Legionella

Alexander Schmidt
contact affiliation	Biozentrum, University of Basel, Basel, Switzerland
contact email	alex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliation	Proteomics Core Facility
contact email	alex.schmidt@unibas.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD015106

PRIDE project URI

• PRIDE
  1. PXD015106
     1. Label: PRIDE project
     2. Name: Quorum sensing elicits functionally distinct subpopulations of infectious Legionella