PXD015089

PXD015089 is an original dataset announced via ProteomeXchange.

Title	Proteomics of non-host response in barley
Description	Nonhost resistance, a resistance of plant species against all nonadapted pathogens, is considered the most durable and efficient immune system in plants. To increase our understanding of the response of barley (Hordeum vulgare L.) plants to infection by powdery mildew, Blumeria graminis f. sp. Tritici, we used quantitative proteomic analysis (LC-MS/MS). We compared the response of two genotypes of barley cultivar Golden Promise, wild type (WT) and plants with overexpression of phytoglobin (previously hemoglobin) class 1 (HO), which has previously been shown to significantly weaken non-host resistance. A total of 8804 proteins were identified and quantified, out of which the abundance of 1044 proteins changed significantly in at least one of the four comparisons (‘i’ stands for ‘inoculated’)- HO/WT and HOi/WTi (giving genotype differences), and WTi/WT and HOi/HO (giving treatment differences). Among these differentially abundant proteins (DAP) were proteins related to structural organization, disease/defense, metabolism, transporters, signal transduction and protein synthesis. More proteins changed in abundance in WT than in HO after inoculation and most DAP increased in abundance.
HostingRepository	PRIDE
AnnounceDate	2020-08-18
AnnouncementXML	Submission_2020-08-18_03:59:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adelina Rogowska-Wrzesinska
SpeciesList	scientific name: Hordeum vulgare; NCBI TaxID: 4513; scientific name: Blumeria graminis f. sp. tritici; NCBI TaxID: 62690;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-20 06:03:44	ID requested
⏵ 1	2020-08-18 03:59:41	announced

Andrzejczak OA, S, ø, rensen CK, Wang WQ, Kovalchuk S, Hagensen CE, Jensen ON, Carciofi M, Hovm, ø, ller MS, Rogowska-Wrzesinska A, M, ø, ller IM, Hebelstrup KH, The effect of phytoglobin overexpression on the plant proteome during nonhost response of barley (Hordeum vulgare) to wheat powdery mildew (Blumeria graminis f. sp. tritici). Sci Rep, 10(1):9192(2020) [pubmed]

submitter keyword: barley, mildew, proteomics, phytoglobin/hemoglobin overexpression

Adelina Rogowska-Wrzesinska
contact affiliation	Department of Biochemistry & Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark
contact email	adelinar@bmb.sdu.dk
lab head
Adelina Rogowska-Wrzesinska
contact affiliation	University of Southern Denmark
contact email	adelinar@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD015089

PRIDE project URI

• PRIDE
  1. PXD015089
     1. Label: PRIDE project
     2. Name: Proteomics of non-host response in barley