PXD015088

PXD015088 is an original dataset announced via ProteomeXchange.

Title	Cucumber mosaic virus resistance: Comparative proteomics of contrasting Cucumis sativus cultivars after long-term infection
Description	Plant viruses are a major threat for a wide range of host species, causing substantial losses in agriculture. Particularly, Cucumber mosaic virus (CMV) evokes severe symptoms, thus dramatically limiting yield. Activation of plant immunity is associated with changes in the gene expression and consequently, cellular proteome to ensure virus resistance. Proteomics proved to be an extremely valuable tool for discovering multiple targets for the rational design of plant protection strategies. Herein, we studied two cultivars of cucumber (Cucumis sativus) resistant ´Heliana´ and susceptible ´Vanda´. Plant cotyledons were mechanically inoculated with CMV isolate PK1, and systemic leaves were harvested at 33 days post-inoculation. Upon protein extraction and filter-aided sample preparations, peptides were profiled by ultrahigh-performance liquid chromatography and comprehensively quantified by ion mobility enhanced mass spectrometry. From 1,516 reproducibly quantified proteins using label-free approach, 133 were differentially abundant among genotypes or treatments by strict statistic and effect size criteria. Pigments and hydrogen peroxide measurements corroborated proteomic findings. Advanced bioinformatics revealed a modular network of affected host proteins. Direct comparison of both genotypes in the uninfected state highlighted more abundant photosynthetic and development-related proteins in resistant cucumber cultivar. Long-term CMV infection showed worse preservation of energy processes and less robust translation in susceptible cultivar versus resistant genotype. Contrary, susceptible cultivar had numerous more abundant stress and defense-related proteins. We proposed promising targets for functional validation in transgenic lines a step toward durable virus resistance in cucurbits and other crops.
HostingRepository	PRIDE
AnnounceDate	2020-01-09
AnnouncementXML	Submission_2020-01-09_02:31:34.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015088
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Maksym Danchenko
SpeciesList	scientific name: Cucumber mosaic virus; NCBI TaxID: 12305; scientific name: Cucumis sativus; NCBI TaxID: 3659;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-20 04:56:01	ID requested
⏵ 1	2020-01-09 02:31:35	announced

Nov, á, kov, á S, Š, ubr Z, Kov, á, č A, Fialov, á I, Beke G, Danchenko M, Cucumber mosaic virus resistance: Comparative proteomics of contrasting Cucumis sativus cultivars after long-term infection. J Proteomics, 214():103626(2020) [pubmed]

submitter keyword: CMV, Label-free quantification, Oxidative stress, Protein network, Photosynthesis, Electron transport

Andrej Kovac
contact affiliation	Institute of Neuroimmunology, Slovak Academy of Sciences, Slovak Republic
contact email	andrej.kovac@savba.sk
lab head
Maksym Danchenko
contact affiliation	Plant Science and Biodiversity Center
contact email	maxymdan@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015088
     1. Label: PRIDE project
     2. Name: Cucumber mosaic virus resistance: Comparative proteomics of contrasting Cucumis sativus cultivars after long-term infection