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PXD015082

PXD015082 is an original dataset announced via ProteomeXchange.

Dataset Summary
Titleidentification of the endogenous insoluble host targets of the Salmonella effectors SSeK3
DescriptionStrains of Salmonella utilise two distinct type three secretion systems to deliver effector proteins directly into host cells. The Salmonella effector SseK3 is a arginine glycosyltransferase that modify mammalian proteins with N-acetyl glucosamine (GlcNAc). Here, we used Arg-GlcNAc glycopeptide immunoprecipitation and mass spectrometry appraoch to identify host proteins GlcNAcylated by endogenous levels of SseK3 during Salmonella infection. Focusing on the insoluble proteome we demostrate multiple typically insoluble proteins are targetted by SseK3 during infections. Surpisingly unlike SseK1 and the EPEC homologue NleB1 which target death domain contain proteins SseK3 appears to modify a number of proteins lacking death domains. These results demostrate that under endogenous infection non-death domain containing proteins can be modified by Salmonella.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:09:45.219.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNichollas Scott
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListcomplex glycosylation
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-20 01:49:54ID requested
12020-07-27 23:50:35announced
22024-10-22 05:09:45announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: glycosylation,Salmonella, proteomics, Arg-GlcNAc, SseK3
Contact List
Elizabeth L Hartland
contact affiliationCentre for Innate Immunity and Infectious Diseases, HudsonInstitute of Medical Research, Clayton, Victoria, Australia
contact emailelizabeth.hartland@hudson.org.au
lab head
Nichollas Scott
contact affiliationUniversity of Melbourne
contact emailnichollas.scott@unimelb.edu.au
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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