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PXD015081

PXD015081 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe host exosome biogenesis pathway is essential for human cytomegalovirus virion assembly and egress
DescriptionHuman Cytomegalovirus (HCMV) infects over 50% of humans, is a leading cause of congenital birth defects, and poses serious risks for immuno-compromised individuals. To expand the molecular knowledge governing virion assembly and egress, we initiated a proteomics-based investigation and identified a significant proportion of host exosome constituents in HCMV virions. Quantitative analysis of exosomes released from uninfected cells (765 proteins) revealed that over 99% of the protein cargo was subsequently integrated into virions, confirming near-complete acquisition of exosome biogenesis during infection. We confirmed VAMP3 is essential for viral trafficking and release of infectious progeny, further validating the intrinsic exploitation of the pathway by HCMV. Therefore, host exosomes serve as the molecular template for virion cargo addition, and provide an export mechanism for virion egress. Our discovery underpins future investigation of host exosome proteins as important modulators of viral maturation, and enhances the understanding of HCMV envelopment, trafficking, fusion and release.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:19:10.882.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRommel Mathias
SpeciesList scientific name: Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5); NCBI TaxID: 10360; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonomethylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos; Orbitrap Fusion ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-20 01:39:13ID requested
12021-02-21 23:24:31announced
22024-10-22 05:19:14announced2024-10-22: Updated project metadata.
Publication List
10.7554/elife.58288;
Turner DL, Korneev DV, Purdy JG, de Marco A, Mathias RA, The host exosome pathway underpins biogenesis of the human cytomegalovirus virion. Elife, 9():(2020) [pubmed]
Keyword List
submitter keyword: VAMP3, herpesvirus, proteomics, exosome, extracellular vesicle, CMV, viral infection, egress, virion assembly,Human cytomegalovirus
Contact List
Rommel Mathias
contact affiliationInfection and Immunity Program Biomedicine Discovery Institute Monash University 23 Innovation Walk Clayton VIC 3800 Australia
contact emailrommel.mathias@monash.edu
lab head
Rommel Mathias
contact affiliationMonash University
contact emailrommel.mathias@monash.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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