PXD015068

PXD015068 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics of mouse cardiomyocytes reveals signalling pathways triggered by a vasodilator cryptide
Description	Animal venoms are natural sources of bioactive molecules such as cryptides that consist in biological active peptides released after proteolytic cleavage of a peptide or protein precursor. The KPP is a cryptided derived from the c-terminal of an hypotensin (TsHpI) a peptide isolated from the venom of Tityus serrulatus scorpion. Previous studies of our group in rodent model demonstrated that KPP maintained the cardioprotective properties of its precursor. Nonetheless, the molecular machinery involved in the cellular response is poorly described to date. Thus, in order to characterize the signalling pathways triggered by KPP we incubate mouse cardiomyocytes with KPP during 5 or 30 min and explore the cell proteome and phosphoproteome by dimethyl labelling quantitation.
HostingRepository	PRIDE
AnnounceDate	2020-07-06
AnnouncementXML	Submission_2020-07-05_22:21:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thiago Verano-Braga
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-19 00:51:47	ID requested
⏵ 1	2020-07-05 22:21:26	announced

Gom, é, z-Mendoza DP, Lemos RP, Jesus ICG, Gorshkov V, McKinnie SMK, Vederas JC, Kjeldsen F, Guatimosim S, Santos RA, Pimenta AMC, Verano-Braga T, Moving Pieces in a Cellular Puzzle: A Cryptic Peptide from the Scorpion Toxin Ts14 Activates AKT and ERK Signaling and Decreases Cardiac Myocyte Contractility via Dephosphorylation of Phospholamban. J Proteome Res, 19(8):3467-3477(2020) [pubmed]

submitter keyword: Cryptide, Cardiomyocytes, Hipotensins, Phosphopreteomics, Venome, Scorpion

Thiago Verano-Braga
contact affiliation	Departamento de Fisiologia e Biofisica, Universidade Federal de Minas Gerais
contact email	tvbraga@icb.ufmg.br
lab head
Thiago Verano-Braga
contact affiliation	Federal University of Minas Gerais
contact email	npf.ufmg@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD015068

PRIDE project URI

• PRIDE
  1. PXD015068
     1. Label: PRIDE project
     2. Name: Phosphoproteomics of mouse cardiomyocytes reveals signalling pathways triggered by a vasodilator cryptide