PXD015066

PXD015066 is an original dataset announced via ProteomeXchange.

Title	The leaves of creeping bentgrass (Agrostis stolonifera) LC−ESI−MS/MS
Description	The global emergence of soil salinization poses a serious challenge to many countries and regions. γ-Aminobutyric acid (GABA) is involved in systemic regulation of plant adaptation to salt stress, but the underling molecular and metabolic mechanism still remains largely unknown. The elevated endogenous GABA level by exogenous application of GABA could significantly improve salt tolerance in creeping bentgrass with the enhancement of antioxidant capacity, photosynthetic characteristics, osmotic adjustment (OA), and water use efficiency. GABA strongly upregulated transcript levels of AsPPa2, AsATPaB2, AsNHX2/4/6, and AsSOS1/20 in roots involved in enhanced capacity of Na+ compartmentalization and mitigation of Na+ toxicity in cytosol. Significant downregulation of AsHKT1/4 expression could be induced by GABA in leaves in relation to maintenance of significantly lower Na+ accumulation and higher K+/Na+ ratio. GABA-depressed aquaporins (AQPs) expression and accumulation induced declines in stomatal conductance and transpiration, thereby reducing water loss in leaves during salt stress. For metabolic regulation, GABA primarily enhanced sugars and amino acids accumulation and metabolism largely contributing to improved salt tolerance through maintaining OA and metabolic homeostasis. Other major pathways could be responsible for GABA-induced salt tolerance including increases in antioxidant defense, heat shock proteins, dehydrins, and myo-inositol accumulation in leaves. Integrative analyses of molecular, protein, metabolic, and physiological changes reveal systemic function of GABA on regulating ions, water, and metabolic homeostasis in non-halophytic creeping bentgrass under salt stress.
HostingRepository	PRIDE
AnnounceDate	2020-01-13
AnnouncementXML	Submission_2020-01-17_06:13:49.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhou Li
SpeciesList	scientific name: Agrostis stolonifera; NCBI TaxID: 63632;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-19 00:46:15	ID requested
1	2020-01-13 04:52:20	announced
⏵ 2	2020-01-17 06:13:50	announced	2020-01-17: Updated publication reference for PubMed record(s): 31916766.

Li Z, Cheng B, Zeng W, Zhang X, Peng Y, -Aminobutyric Acid in Regulating Ionic, Water, and Metabolic Homeostasis in Creeping Bentgrass under Salt Stress. J Proteome Res, 19(2):769-780(2020) [pubmed]

submitter keyword: bentgrass, proteomics, LC−ESI−MS/MS, salt stress

Yan Peng
contact affiliation	Sichuan Agricultural University
contact email	pengyanlee@163.com
lab head
Zhou Li
contact affiliation	Sichuan Agricultural University
contact email	lizhou1986814@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015066
     1. Label: PRIDE project
     2. Name: The leaves of creeping bentgrass (Agrostis stolonifera) LC−ESI−MS/MS