PXD015061

PXD015061 is an original dataset announced via ProteomeXchange.

Title	Proteome response of the diatom Phaeodactylum tricornutum to short term shifts in nitrogen source and availability.
Description	The photosynthetic model marine diatom Phaeodactylum tricornutum was cultured with varying nitrogen sources and availability to elicit shifts in the proteome. Cells were grown to mid-exponential phase on ammonium (880 uM), collected by centrifugation and resuspended in N-free media for 2 hrs, and spiked with nitrate (150 uM) for 90 min. Cells were again collected by centrifugation, washed in N-free media, and resuspended in experimental treatments with no nitrogen (N-) and a nitrogen source (300 uM) provided as ammonium, nitrite, or nitrate. Each experimental treatment was sampled after 15 min, 45 min, and 18 hr.
HostingRepository	PRIDE
AnnounceDate	2019-11-27
AnnouncementXML	Submission_2019-11-27_06:52:02.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015061
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew Monroe
SpeciesList	scientific name: Phaeodactylum tricornutum; NCBI TaxID: 2850;
ModificationList	Oxidation; iTRAQ8plex; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-16 05:36:24	ID requested
1	2019-09-25 07:14:11	announced
2	2019-10-11 00:04:19	announced	2019-10-11: Updated publication reference for PubMed record(s): 31591397.
⏵ 3	2019-11-27 06:52:04	announced	2019-11-27: Updated project metadata.

Smith SR, Dupont CL, McCarthy JK, Broddrick JT, Oborn, í, k M, Hor, á, k A, F, ü, ssy Z, Cihl, á, ř J, Kleessen S, Zheng H, McCrow JP, Hixson KK, Ara, ú, jo WL, Nunes-Nesi A, Fernie A, Nikoloski Z, Palsson BO, Allen AE, Evolution and regulation of nitrogen flux through compartmentalized metabolic networks in a marine diatom. Nat Commun, 10(1):4552(2019) [pubmed]

submitter keyword: nitrogen, nitrate, nitrite, ammonium, iron, diel, diatom

Andrew E. Allen
contact affiliation	Scripps Institution of Oceanography, University of California
contact email	aallen@ucsd.edu
lab head
Matthew Monroe
contact affiliation	Pacific Northwest National Laboratory
contact email	matthew.monroe@pnnl.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD015061

PRIDE project URI

• PRIDE
  1. PXD015061
     1. Label: PRIDE project
     2. Name: Proteome response of the diatom Phaeodactylum tricornutum to short term shifts in nitrogen source and availability.