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PXD015061

PXD015061 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome response of the diatom Phaeodactylum tricornutum to short term shifts in nitrogen source and availability.
DescriptionThe photosynthetic model marine diatom Phaeodactylum tricornutum was cultured with varying nitrogen sources and availability to elicit shifts in the proteome. Cells were grown to mid-exponential phase on ammonium (880 uM), collected by centrifugation and resuspended in N-free media for 2 hrs, and spiked with nitrate (150 uM) for 90 min. Cells were again collected by centrifugation, washed in N-free media, and resuspended in experimental treatments with no nitrogen (N-) and a nitrogen source (300 uM) provided as ammonium, nitrite, or nitrate. Each experimental treatment was sampled after 15 min, 45 min, and 18 hr.
HostingRepositoryPRIDE
AnnounceDate2019-11-27
AnnouncementXMLSubmission_2019-11-27_06:52:02.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD015061
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMatthew Monroe
SpeciesList scientific name: Phaeodactylum tricornutum; NCBI TaxID: 2850;
ModificationListOxidation; iTRAQ8plex; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-16 05:36:24ID requested
12019-09-25 07:14:11announced
22019-10-11 00:04:19announced2019-10-11: Updated publication reference for PubMed record(s): 31591397.
32019-11-27 06:52:04announced2019-11-27: Updated project metadata.
Publication List
Smith SR, Dupont CL, McCarthy JK, Broddrick JT, Oborn, í, k M, Hor, á, k A, F, ü, ssy Z, Cihl, á, ř J, Kleessen S, Zheng H, McCrow JP, Hixson KK, Ara, ú, jo WL, Nunes-Nesi A, Fernie A, Nikoloski Z, Palsson BO, Allen AE, Evolution and regulation of nitrogen flux through compartmentalized metabolic networks in a marine diatom. Nat Commun, 10(1):4552(2019) [pubmed]
Keyword List
submitter keyword: nitrogen, nitrate, nitrite, ammonium, iron, diel, diatom
Contact List
Andrew E. Allen
contact affiliationScripps Institution of Oceanography, University of California
contact emailaallen@ucsd.edu
lab head
Matthew Monroe
contact affiliationPacific Northwest National Laboratory
contact emailmatthew.monroe@pnnl.gov
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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