PXD015061
PXD015061 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome response of the diatom Phaeodactylum tricornutum to short term shifts in nitrogen source and availability. |
Description | The photosynthetic model marine diatom Phaeodactylum tricornutum was cultured with varying nitrogen sources and availability to elicit shifts in the proteome. Cells were grown to mid-exponential phase on ammonium (880 uM), collected by centrifugation and resuspended in N-free media for 2 hrs, and spiked with nitrate (150 uM) for 90 min. Cells were again collected by centrifugation, washed in N-free media, and resuspended in experimental treatments with no nitrogen (N-) and a nitrogen source (300 uM) provided as ammonium, nitrite, or nitrate. Each experimental treatment was sampled after 15 min, 45 min, and 18 hr. |
HostingRepository | PRIDE |
AnnounceDate | 2019-11-27 |
AnnouncementXML | Submission_2019-11-27_06:52:02.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD015061 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Matthew Monroe |
SpeciesList | scientific name: Phaeodactylum tricornutum; NCBI TaxID: 2850; |
ModificationList | Oxidation; iTRAQ8plex; Carbamidomethyl |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-08-16 05:36:24 | ID requested | |
1 | 2019-09-25 07:14:11 | announced | |
2 | 2019-10-11 00:04:19 | announced | 2019-10-11: Updated publication reference for PubMed record(s): 31591397. |
⏵ 3 | 2019-11-27 06:52:04 | announced | 2019-11-27: Updated project metadata. |
Publication List
Smith SR, Dupont CL, McCarthy JK, Broddrick JT, Oborn, í, k M, Hor, á, k A, F, ü, ssy Z, Cihl, á, ř J, Kleessen S, Zheng H, McCrow JP, Hixson KK, Ara, ú, jo WL, Nunes-Nesi A, Fernie A, Nikoloski Z, Palsson BO, Allen AE, Evolution and regulation of nitrogen flux through compartmentalized metabolic networks in a marine diatom. Nat Commun, 10(1):4552(2019) [pubmed] |
Keyword List
submitter keyword: nitrogen, nitrate, nitrite, ammonium, iron, diel, diatom |
Contact List
Andrew E. Allen | |
---|---|
contact affiliation | Scripps Institution of Oceanography, University of California |
contact email | aallen@ucsd.edu |
lab head | |
Matthew Monroe | |
contact affiliation | Pacific Northwest National Laboratory |
contact email | matthew.monroe@pnnl.gov |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD015061 |
PRIDE project URI |
Repository Record List
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