PXD015051

PXD015051 is an original dataset announced via ProteomeXchange.

Title	Identification of an RNA sponge that controls the levels, processing and efficacy of the RoxS riboregulator of central metabolism in B. subtilis
Description	Only a few small regulatory RNAs (sRNAs) have been characterized in B. subtilis, the paradigm of Gram-positive bacteria, and one of the major challenges is target identification. Here we use global in vivo RNA psoralen cross-linking to identify RNA-RNA partners in Bacillus subtilis. Two sRNAs, RoxS and FsrA, play key roles in balancing the metabolic state of the cell in response to carbon sources and iron limitation, respectively. In this study, we identify new mRNA targets for both RoxS and FsrA, and a small RNA (S345/RosA) that is able to interact with both sRNAs. We report that RosA controls the maturation and degradation of RoxS and acts as a sponge to limit the efficacy of RoxS on its targets. Expression of RosA is catabolically repressed by the transcription factor CcpA. We provide evidence that the RosA/RoxS interaction plays a key role in regulating metabolism in response to a switches in carbon source.
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_13:48:04.402.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Ramon Hernandez-Fernaud
SpeciesList	scientific name: Bacillus subtilis; NCBI TaxID: 1423;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-16 02:31:54	ID requested
⏵ 1	2021-09-08 13:48:05	announced

Durand S, Callan-Sidat A, McKeown J, Li S, Kostova G, Hernandez-Fernaud JR, Alam MT, Millard A, Allouche D, Constantinidou C, Condon C, Denham EL, Identification of an RNA sponge that controls the RoxS riboregulator of central metabolism in Bacillus subtilis. Nucleic Acids Res, 49(11):6399-6419(2021) [pubmed]

submitter keyword: Bacillus subtilis, RNAs, metabolism, RosA, RoxS

Emma L. Denham
contact affiliation	Senior Lecturer Department of Biology and Biochemistry University of Bath Claverton Down Bath BA2 7AY
contact email	eld49@bath.ac.uk
lab head
Juan Ramon Hernandez-Fernaud
contact affiliation	FIISC
contact email	jrfernaud@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015051
     1. Label: PRIDE project
     2. Name: Identification of an RNA sponge that controls the levels, processing and efficacy of the RoxS riboregulator of central metabolism in B. subtilis