PXD015045

PXD015045 is an original dataset announced via ProteomeXchange.

Title	Shotgun proteomics of Roseburia grown on human milk oligosaccharides and of Roseburia grown on mucin in co-culture with Akkermansia muciniphilia.
Description	The main objective of the present proteomic study is to identify the metabolic response, in particular theglycan uptake and degradation machinery, conferring members of Roseburia growth on HMOs and/or onrelated O-glycans. Accordingly, the proteomes of R. hominis and R. inulinivorans bothgrown on humanmilk oligosaccharides (HMOs), were compared to glucose to reveal the molecular basis for growth onHMOs. Furthermore, we compare the proteomes of R. hominis and R. inulinivorans grownin co-culturewith A. muciniphilia either on mucin or on glucose to identify potential metabolic routes of mucin derivedO-glycan utilization in Roseburia.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:08:28.395.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Pichler
SpeciesList	scientific name: Akkermansia muciniphila ATCC BAA-835; NCBI TaxID: 349741; scientific name: Roseburia hominis A2-183; NCBI TaxID: 585394; scientific name: Roseburia inulinivorans DSM 16841; NCBI TaxID: 622312;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-16 01:10:11	ID requested
1	2020-07-05 22:08:09	announced
⏵ 2	2024-10-22 05:08:33	announced	2024-10-22: Updated project metadata.

10.1038/s41467-020-17075-x;

Pichler MJ, Yamada C, Shuoker B, Alvarez-Silva C, Gotoh A, Leth ML, Schoof E, Katoh T, Sakanaka M, Katayama T, Jin C, Karlsson NG, Arumugam M, Fushinobu S, Abou Hachem M, Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways. Nat Commun, 11(1):3285(2020) [pubmed]

submitter keyword: LC-MSMS, Metaproteomics,Roseburia, Proteomics, HMOs, Mucin, Akkermansia muciniphilia, O-glycans

Maher Abou Hachem
contact affiliation	DTU BIOENGINEERING Department of Biotechnology and Biomedicine PCET-Protein Glycoscience and Biotechnology Technical University of Denmark 2800 Kgs. Lyngby
contact email	maha@bio.dtu.dk
lab head
Michael Pichler
contact affiliation	DTU
contact email	mijap@dtu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD015045

PRIDE project URI

• PRIDE
  1. PXD015045
     1. Label: PRIDE project
     2. Name: Shotgun proteomics of Roseburia grown on human milk oligosaccharides and of Roseburia grown on mucin in co-culture with Akkermansia muciniphilia.