PXD015045 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Shotgun proteomics of Roseburia grown on human milk oligosaccharides and of Roseburia grown on mucin in co-culture with Akkermansia muciniphilia. |
Description | The main objective of the present proteomic study is to identify the metabolic response, in particular theglycan uptake and degradation machinery, conferring members of Roseburia growth on HMOs and/or onrelated O-glycans. Accordingly, the proteomes of R. hominis and R. inulinivorans bothgrown on humanmilk oligosaccharides (HMOs), were compared to glucose to reveal the molecular basis for growth onHMOs. Furthermore, we compare the proteomes of R. hominis and R. inulinivorans grownin co-culturewith A. muciniphilia either on mucin or on glucose to identify potential metabolic routes of mucin derivedO-glycan utilization in Roseburia. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:08:28.395.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Michael Pichler |
SpeciesList | scientific name: Akkermansia muciniphila ATCC BAA-835; NCBI TaxID: 349741; scientific name: Roseburia hominis A2-183; NCBI TaxID: 585394; scientific name: Roseburia inulinivorans DSM 16841; NCBI TaxID: 622312; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-08-16 01:10:11 | ID requested | |
1 | 2020-07-05 22:08:09 | announced | |
⏵ 2 | 2024-10-22 05:08:33 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1038/s41467-020-17075-x; |
Pichler MJ, Yamada C, Shuoker B, Alvarez-Silva C, Gotoh A, Leth ML, Schoof E, Katoh T, Sakanaka M, Katayama T, Jin C, Karlsson NG, Arumugam M, Fushinobu S, Abou Hachem M, Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways. Nat Commun, 11(1):3285(2020) [pubmed] |
Keyword List
submitter keyword: LC-MSMS, Metaproteomics,Roseburia, Proteomics, HMOs, Mucin, Akkermansia muciniphilia, O-glycans |
Contact List
Maher Abou Hachem |
contact affiliation | DTU BIOENGINEERING Department of Biotechnology and Biomedicine PCET-Protein Glycoscience and Biotechnology Technical University of Denmark 2800 Kgs. Lyngby |
contact email | maha@bio.dtu.dk |
lab head | |
Michael Pichler |
contact affiliation | DTU |
contact email | mijap@dtu.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD015045 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015045
- Label: PRIDE project
- Name: Shotgun proteomics of Roseburia grown on human milk oligosaccharides and of Roseburia grown on mucin in co-culture with Akkermansia muciniphilia.