PXD015037

PXD015037 is an original dataset announced via ProteomeXchange.

Title	Development of Photocrosslinking Probes Based on Huwentoxin-IV to Map the Site of Interaction on Nav1.7
Description	This study reports on the design of a library of photoprobes based on the potent spider toxin Huwentoxin-IV and the determination of the toxin binding interface on VSD2 of Nav1.7 through a photocrosslinking and tandem mass spectrometry approach. Our Huwentoxin-IV probes selectively crosslink to extracellular loop S1-2 and helix S3 of VSD2 in a chimeric channel system. Sample preparation of purified chimeric channels crosslinked with the photoprobes was based on the FASP protocol. Various digestion enzymes were evaluated in order to maximise the sequence coverage of this membrane protein. Finally, MeroX was employed in order to identify the sites of crosslinking. Our results provide a strategy that will enable mapping of sites of interaction of other ICK peptides on Nav channels.
HostingRepository	PRIDE
AnnounceDate	2019-11-18
AnnouncementXML	Submission_2019-11-18_02:00:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Foteini Tzakoniati
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-15 02:59:28	ID requested
⏵ 1	2019-11-18 02:00:27	announced

Dataset with its publication pending

submitter keyword: Nav1.7, Huwentoxin-IV, photoaffinity labelling, diazirine, peptide mapping

Edward William Tate
contact affiliation	Department of Chemistry, Imperial College London, United Kingdom
contact email	ed.tate@imperial.ac.uk
lab head
Foteini Tzakoniati
contact affiliation	Imperial College London
contact email	ft08@ic.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015037
     1. Label: PRIDE project
     2. Name: Development of Photocrosslinking Probes Based on Huwentoxin-IV to Map the Site of Interaction on Nav1.7