PXD015026

PXD015026 is an original dataset announced via ProteomeXchange.

Title	A spiked human proteomic dataset from human osteogenic differentiated BMSCs and ASCs for use as a spectral library, for modelling pathways as well as protein mapping
Description	This article describes a mass spectrometry data set generated from osteogenic differentiated bone marrow stromal cells (BMSCs) and adipose tissue derived stromal cells (ASCs) of a 24-year old healthy donor spiked with iRT peptides. Cells have been identified via FACS-Analysis positive for CD90 and CD105 and negative for CD14, CD34, CD45 and CD11b and tri-lineage differentiation. Obtaining a sufficient amount of high-quality tissue is the key limiting factor for establishing a region-specific spectral library. Hence, combining existing spectral libraries for data-independent acquisition analysis (DIA) can overcome this major limitation. Moreover, these data can be used to map region-specific proteins and to model region-specific pathways. Both can improve our understanding of the functioning in greater depth. In addition, these data can also be used to determine the optimal settings for measuring proteins and peptides of interest. To create the specific spectral library, the tissue was first homogenized and then fractionated via different types of SDS gel electrophoresis, resulting in 11 fractions. These fractions were analysed by nanoHPLC-ESI-MS/MS, resulting in 24 data files.
HostingRepository	PRIDE
AnnounceDate	2019-11-27
AnnouncementXML	Submission_2020-09-28_00:23:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Annika Guntermann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-14 05:53:04	ID requested
1	2019-10-22 06:42:03	announced
2	2019-11-27 06:00:44	announced	2019-11-27: Updated project metadata.
⏵ 3	2020-09-28 00:23:54	announced	2019-11-27: Updated project metadata. 2020-09-28: Updated publication reference for PubMed record(s): 31763404.

Dadras M, Marcus K, Wagner JM, Wallner C, Becerikli M, Jaurich H, Dittfeld S, Lehnhardt M, Serschnitzki B, Guntermann A, Schilde L, Behr B, May C, A spiked human proteomic dataset from human osteogenic differentiated BMSCs and ASCs for use as a spectral library, for modelling pathways as well as protein mapping. Data Brief, 27():104748(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: osteogenic differentiated mesenchymal stem cells, nanoHPLC-ESI-MS/MS, data-independent acquisition analysis

Caroline May
contact affiliation	Medizinische Proteom-Center 44801 Bochum, Germany
contact email	Caroline.May@ruhr-uni-bochum.de
lab head
Annika Guntermann
contact affiliation	Medizinisches Proteom-Center
contact email	Annika.Guntermann@rub.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015026
     1. Label: PRIDE project
     2. Name: A spiked human proteomic dataset from human osteogenic differentiated BMSCs and ASCs for use as a spectral library, for modelling pathways as well as protein mapping