PXD015025

PXD015025 is an original dataset announced via ProteomeXchange.

Title	Absolute Quantification of Yeast Proteins Using Synthetic Peptide Standards and TMT labeling
Description	The absolute protein amounts for the selected yeast ribosomal proteins (RPs) were determined using the standard peptides and TMT labelling. The range of the standard tryptic peptides were selected and synthesized based on the preliminary data-dependent experiments. The synthetic peptides were mixed and four aliquots containing different amounts of the peptides were labelled each with a different TMT labelling reagent. The yeast cell lysate was digested and labelled in parallel. The labelled cellular and standard samples were combined, and the absolute peptide amounts in the lysate were calculated by comparison to the known amounts in the standard TMT channels.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:00:49.443.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Egor Vorontsov
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-14 05:31:40	ID requested
1	2020-03-13 00:38:49	announced
2	2020-04-26 23:16:22	announced	2020-04-27: Updated publication reference for PubMed record(s): 32312967.
⏵ 3	2024-10-22 05:00:51	announced	2024-10-22: Updated project metadata.

10.1038/s41467-020-15749-0;

Yu R, Campbell K, Pereira R, Bj, ö, rkeroth J, Qi Q, Vorontsov E, Sihlbom C, Nielsen J, Nitrogen limitation reveals large reserves in metabolic and translational capacities of yeast. Nat Commun, 11(1):1881(2020) [pubmed]

submitter keyword: TMT, yeast,Tandem mass tag, standard peptides, Saccharomyces cerevisiae, absolute quantification, synthetic peptides, Orbitrap Fusion

Jens Nielsen
contact affiliation	Professor, Department of Biology and Biological Engineering, Chalmers University of Technology, SE-41296 Gothenburg, Sweden; Wallenberg Center for Protein Research, Chalmers University of Technology, SE-41296 Gothenburg, Sweden; Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, SE-41296 Gothenburg, Sweden; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, DK-2800 Lyngby, Denmark
contact email	nielsenj@chalmers.se
lab head
Egor Vorontsov
contact affiliation	Proteomics Core Facility, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
contact email	yegor.msu@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD015025

PRIDE project URI

• PRIDE
  1. PXD015025
     1. Label: PRIDE project
     2. Name: Absolute Quantification of Yeast Proteins Using Synthetic Peptide Standards and TMT labeling