PXD015021

PXD015021 is an original dataset announced via ProteomeXchange.

Title	Generation of macrophages that are profoundly pro-remyelinating through unexpected activation cues
Description	Excessive inflammation within the central nervous system is injurious, but an immune response is also required for its repair. Macrophages are versatile cells that adopt different properties depending upon their microenvironment. Exposing macrophages to interleukin-4 and -13 (IL4/IL13) has incurred interest for their reparative properties. Unexpectedly, while macrophages exposed to the classic pro-inflammatory signals (interferon-γ/lipopolysaccharide, IFN/LPS) killed neurons and oligodendrocytes in culture, the addition of LPS to IL4/IL13-treated macrophages profoundly elevated IL10, repair metabolites (lactate, ornithine), glucose metabolism and the oligodendrocyte-trophic heparin-binding epidermal growth factor (HBEGF); cells did not display pro-inflammatory or neurotoxic features. In mice with spinal cord demyelination, locally-applied IL4/IL13 was insufficient to alter responses beyond controls; remarkably, the LPS/IL4/IL13-treated animals significantly increased lesional phagocytic macrophages/microglia, lactate and HBEGF levels, oligodendrogenesis and remyelination. We report a remarkably reparative state of macrophages that is unexpectedly generated by the integration of pro- (LPS) and anti- (IL4/IL13) inflammatory activation cues.
HostingRepository	PRIDE
AnnounceDate	2019-09-06
AnnouncementXML	Submission_2019-09-09_02:48:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antoine Dufour
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-14 01:45:47	ID requested
⏵ 1	2019-09-09 02:48:56	announced

Dataset with its publication pending

submitter keyword: Macrophage, Multiple sclerosis, remyelinating

Antoine Dufour
contact affiliation	University of Calgary, Department of Physiology and Pharmacology
contact email	antoine.dufour@ucalgary.ca
lab head
Antoine Dufour
contact affiliation	University of Calgary Department of Physiology and Pharmacology
contact email	antoine.dufour@ucalgary.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD015021

PRIDE project URI

• PRIDE
  1. PXD015021
     1. Label: PRIDE project
     2. Name: Generation of macrophages that are profoundly pro-remyelinating through unexpected activation cues