PXD015010

PXD015010 is an original dataset announced via ProteomeXchange.

Title	Plasma membrane proteome of adhesion-competent endometrial epithelial cells and its modulation by Rab11a
Description	Human endometrial epithelium (EE) is composed of a multitude of proteins, amongst which those localized on the plasma membrane (plasma membrane proteins-PMPs) are of critical relevance in early stages of implantation. Evidences support the key role of few PMPs in implantation. However, many remain unidentified, as efforts have not been made till date to generate the plasma membrane proteome of human EE cells, using a gel-free approach. This study presents a protein catalogue of the PMP enriched fraction of Ishikawa cell line; often used as an in vitro model for embryo-adhesive EE. Liquid Chromatography with tandem mass spectrometry identified 3598 proteins. Of these, 1963 proteins were annotated for their membrane localization. Of 1963 proteins, 1321 were found to have transmembrane domain and 43 proteins had GPI anchor. Extensive data mining revealed endometrial expression of 943 proteins reported in human and/or rodents. Further, quantitative alterations were observed in the plasma membrane proteome on perturbation of intracellular trafficking. Silencing of Rab11a (known for its role in plasma membrane organization) expression caused alteration in the abundance of 74 proteins. Caveolin-1 and EpCAM levels were reduced whereas Rab4a abundance increased in the PMP extracts of Rab11a-deficient cells, compared to control cells. Briefly, the study reports the identity of several novel plasma membrane-localized proteins. A major spin-off of the study is the identification of novel proteins trafficked by Rab11a to the plasma membrane. Targeted analysis of novel PMPs may reveal their specific roles in endometrial receptivity and implantation.
HostingRepository	PRIDE
AnnounceDate	2019-11-08
AnnouncementXML	Submission_2020-01-14_06:20:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Geetanjali Sachdeva
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-13 02:45:24	ID requested
1	2019-11-08 06:53:59	announced
⏵ 2	2020-01-14 06:20:29	announced	2020-01-14: Updated publication reference for PubMed record(s): 31737970.

Kakar-Bhanot R, Brahmbhatt K, Kumar V, Suryawanshi AR, Srivastava S, Chaudhari U, Sachdeva G, Plasma membrane proteome of adhesion-competent endometrial epithelial cells and its modulation by Rab11a. Mol Reprod Dev, 87(1):17-29(2020) [pubmed]

submitter keyword: Endometrium, Epithelium, Plasma membrane, Proteomics, Ishikawa

Prof. Geetanjali Sachdeva
contact affiliation	Group Leader, Primate Biology, National Institute for Research in Reproductive Health, India
contact email	sachdevag@nirrh.res.in
lab head
Geetanjali Sachdeva
contact affiliation	Ph.D.
contact email	sachdevag@nirrh.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD015010

PRIDE project URI

• PRIDE
  1. PXD015010
     1. Label: PRIDE project
     2. Name: Plasma membrane proteome of adhesion-competent endometrial epithelial cells and its modulation by Rab11a